_Experiment 2._ A guineapig was placed in the same jar, and twenty-two and a half grains of chloroform were introduced in the same manner as before, being three-quarters of a grain to each hundred cubic inches of air. In two or three minutes the chloroform was converted into vapour and diffused through the air in the jar. Six minutes afterwards the guineapig began to stagger, and soon afterwards was unable to keep on its legs. It was kept for half an hour in the jar, but did not become further affected. It was occasionally quiet, but always tried to walk when disturbed by moving the jar. When taken out, it flinched on being pricked, and it tried to walk, although unable to support itself on its legs. It recovered from the effects of the chloroform in three or four minutes. _Experiment 3._ Another guineapig was placed in the same jar, and thirty grains of chloroform were introduced. In three or four minutes the chloroform had evaporated and diffused itself through the air in the jar. In two minutes afterwards the guineapig lay down, but stirred when the jar was moved. It was allowed to remain in the jar for half an hour, being asleep except when disturbed. When taken out it awoke and endeavoured to walk, but was unable to support itself at first. It flinched on being pricked. Recovered in three or four minutes. _Experiment 4._ A cat was placed in the same jar, and the same quantity of chloroform was introduced. In three minutes it had evaporated, and, when ten minutes had elapsed, the cat began to stagger. Soon afterwards it was unable to support itself on its legs; and, when a quarter of an hour had elapsed, it seemed asleep, but raised its head when the jar was inclined. It remained apparently sleeping till the end of an hour, when it was taken out. It was unable to stand, but was sensible to the slightest pinch. It recovered gradually in the course of twenty minutes. _Experiment 5._ Sixteen grains of chloroform were put into a jar holding 1,600 cubic inches, being one grain to each 100 cubic inches of air, as in the two last experiments. When it was converted into vapour, a white mouse was introduced, by moving the lid a little to one side for a moment. After running about for a minute and a half, it began to stagger and to lose power, but it continued to run with little intermission till three minutes had expired. After this it lay still, except when the jar was inclined, when it always endeavoured to maintain its balance and to walk. It also moved its limbs now and then, when not disturbed. It was removed at the end of ten minutes, when it tried to walk, but was unable. It did not flinch when its tail was pinched, but flinched, half a minute after its removal, on one of its toes being pinched. It recovered in two or three minutes.