It was now put into the jar again, and the same quantity of chloroform was introduced in the same manner as before. In five minutes, it had evaporated, and the cat was again insensible. In other five minutes, it was breathing rather deeply, forty-eight times in the minute. Twelve minutes after the chloroform had evaporated, the cat was breathing in the same manner, but sixty-eight times in the minute. The breathing afterwards became shallow and feeble, and half an hour after the chloroform had evaporated, it was eighty-eight in the minute. In five minutes more, the breathing ceased. The cat was taken out of the jar, and the stethoscope was applied to the chest. The heart could not be heard to beat at first, but in a short time, the cat gave a gasp, and the heart’s action returned, and the breathing became reestablished. _Experiment 16._ Forty-eight grains of chloroform were put into a jar holding 1,600 cubic inches, being three grains to each hundred cubic inches, and when it was converted into vapour and diffused through the air in the jar, a white mouse was introduced. It ran about for a minute and a half, and then it was unable to move any longer. In nine minutes and a half, the breathing was getting very slow, and the mouse was immediately removed. Before it could be got out, it appeared to have entirely ceased to breathe, but it immediately began to gasp at intervals. In a minute, the breathing improved, and soon became natural. Three minutes after its removal, the mouse began to walk in a ring, and in seven minutes, it had quite recovered. _Experiment 17._ A guineapig was placed in a jar holding 1,600 cubic inches, and fifty-four grains of chloroform were introduced on blotting paper, in the manner previously explained; being three grains and three-eighths to each hundred cubic inches of air. In four minutes, the chloroform had evaporated, and become diffused through the air in the jar, and the guineapig was drowsy. In three minutes more, it was lying apparently insensible, and breathing feebly, thirty times in the minute. After this, it kicked occasionally for a few minutes. When six minutes had elapsed from the time when the chloroform had evaporated, the breathing was very feeble, and apparently performed only by the diaphragm. From fifteen to eighteen minutes, I could hardly see whether the animal was breathing or not, and it was taken out of the glass at eighteen minutes, when the breathing appeared to have ceased. The stethoscope was applied to the chest, and the sounds of the heart could scarcely be heard at first, but the breathing returned in a few seconds, and the action of the heart improved. The guineapig gradually recovered, but it was not able to walk for twenty-five minutes.