When a loop is carefully emptied and suspended in a solution of m/8-m/6 NaCl in the way described, it is found that after a considerable time (20-40 minutes) it is still quite empty. If, on the other hand, an m/2 solution of NaCl is used the loop is found to contain after 15 to 20 minutes a small but distinct quantity of clear yellowish fluid resembling the normal intestinal juice. A loop 27 cm. long suspended in m/2 NaCl for 20 minutes contained 0.6 c.c. fluid. A second loop 30 cm. long in the same solution contained 0.8 c.c. fluid. A control loop of the same length in m/8 NaCl remained empty. In m/2 NaCl the movements of the loop are spasmodic and the contractions very strong. The movements do not usually last more than 5 minutes, although the loop may be removed from this solution 15 minutes after the movements have ceased, and may be caused to move again by immersing it in m/8 NaCl containing ¹⁄₂₅ of its volume of m/8 BaCl_{2}. This shows that the loop is not actually killed by the strong NaCl solution. A loop suspended in m/6 sodium citrate solution showed active peristaltic movements, lasting for 20 to 30 minutes. No fluid, however, collected in this loop. When suspended, however, in m/2 sodium citrate a measurable quantity of fluid was obtained after 20 minutes. A similar result was obtained with Na_{2}SO_{4}, active peristalsis, but no secretion being caused in an m/6 solution. In an m/2 solution, however, a considerable quantity of fluid collected in the loop. A loop 28 cm. long suspended in m/8 NaF exhibited active peristaltic movements which continued for less than 10 minutes. At the end of 20 minutes the loop was found to contain 0.8 c.c. of a clear but slightly blood-stained fluid. Loops suspended in m/6 NaCl containing m/6 or m/8 BaCl_{2} in the proportion of 50 to 1 or 70 to 1 showed very strong muscular contractions and a well marked secretion of fluid. The muscular movements were characteristic of barium. Violent local contractions and firm constrictions of the intestine together with strong peristaltic movements took place. The loops always contained a distinct and measurable quantity of fluid after being suspended in this fluid, as may be seen in the table which follows. Loops placed in m/6 CaCl_{2} showed no muscular movements whatever, nor did any fluid gather in the lumen. This is in marked contrast to the behavior of the loops in the solutions already described. In equal parts of m/6 NaCl and m/6 CaCl_{2} no movements or secretion took place. The results of a number of these experiments may be observed in the following table:--