Here the sugar appeared in the intestinal juice after about 200 c.c. NaCl solution had been injected. The injection was made in each case into the vein of the ear by means of a pressure bottle connected with the water tap. The pressure bottle was in turn connected with a bottle holding the solution, which was thus forced out at a constant rate through a long rubber tube immersed in water at 40° C. A hypodermic needle was fastened in the end of this tube and inserted into the marginal vein of the rabbit’s ear. In this way the quantity of fluid injected could be accurately measured and controlled. The salt solution after passing through the long tube reached the ear at approximately body temperature. It is of the greatest importance to protect the intestinal loops in every way possible from loss of heat or from drying. In the above experiment the _intestinal glycosuria_, if such a term may be applied to this phenomenon, appears under circumstances which are exactly the same as those necessary for the production of sugar in the urine by saline infusions. A further example of this is shown in the following experiment:-- Rabbit--Blood vessels of both kidneys ligatured. Intestinal loop including duodenum 32 cm. long. In the loop were found 3 c.c. fluid which contained no sugar. NaCl m/6 Intestinal Sugar examination of Time injected juice intestinal juice 9:45 Infusion begun Loop emptied, No sugar 3 c.c. 10:15 10 c.c. 3 c.c. No sugar 10:45 70 c.c. 3 c.c. No sugar 11:15 75 c.c. 3.2 c.c. No sugar 11:45 85 c.c. 5 c.c. Sugar abundant } 0.202% } 0.222% 12:15 80 c.c. 9.5 c.c. Sugar abundant 0.25% 12:45 150 c.c. 21 c.c. Sugar abundant -------- 470 c.c. Here the sugar appeared after the infusion of about 240 c.c. NaCl solution. The experiment was not carried on to see how long the sugar would continue to be present in the intestinal juice. The animal was killed, and it was found that the remaining loops of the small intestine held 32 c.c. of fluid which contained sugar. The stomach contents included about 40 c.c. fluid which also contained sugar. Of the 470 c.c. of fluid injected, 78.7 c.c. were eliminated by the small intestine and 40 by the stomach. The alimentary canal, then, exclusive of the large intestine, eliminated about 118 c.c. of the fluid introduced, which is approximately 25%.