Physiology and histology of the Cubomedusæ: including Dr. F.S. Conant's notes on the physiologyBerger, E. W. (Edward William)
Science
Physiology and histology of the Cubomedusæ: including Dr. F.S. Conant's notes on the physiology
Berger, E. W. (Edward William)
Cubomedusae
The walls and axis of these nuclei have the structure of a very fine and
dense network that stains very dark with iron-hæmatoxylin. It stains
quite like the reticulum of any nucleus, but is very dense, as though
all the reticulum of the nucleus had been crowded together at the
surface. Judging from appearances like p (Fig. 20), the hollowing out, so
to speak, of these nuclei, would seem to be a process of vacuolation, the
reticulum becoming crowded aside to the surface. But how, on this view,
to amount for the formation of the axis, I do not know. Perhaps the axis
is formed by a pushing in of two opposite poles of a nucleus, the two
invaginations meeting and fusing. On this supposition one might expect
the axis to be hollow (cylindrical), but I could not determine that it
was. Perhaps the centrosphere (or spheres) (see the next paragraph) has
something to do with the formation of the axis (Fig. 20, b, g, e, etc.).
In the nuclei of Fig. 20 with the dark outlines, and of Fig. 7 a small
reticular body is seen just opposite one end of the axis, or opposite
both ends in g. In d (Fig. 20) this body is seen next the axis just
below (outside) the hollow cup represented by the hollow ring. In this
instance a central granule is seen in the reticular body, as also in
c. I take this reticular body to be the centrosphere, and the central
granule in c and d the centrosome. In k, l, m, n, and o (Fig. 20), which
are from another series, in which the walls of the nuclei did not stain
so dark as in the other nuclei of the same figure, a nucleolus could be
definitely seen, indeed, sometimes quite perched upon the wall of the
nucleus (k, l). In several instances I could see two nuclei, as in o. But
besides these nucleoli, I could in several instances see quite definitely
a reticular body (centrosphere) opposite the axis (m, n, o) quite as I
described for the nuclei with the dark outlines. In a, b, c, d, e and g
the nuclei could not be so readily demonstrated, but I could occasionally
see a darker stained body as in a, c and g, that I have no doubt is the
nucleolus, which here, again, is perched quite upon the surface of the
nucleus. This position of the nucleolus is perhaps due to its having been
crowded to one side by the nucleus becoming hollow. It is no uncommon
thing, either, to find several nuclei in a single cell, sometimes in
process of division or just divided as o and e (Fig. 20), also h, i and
j. The whole nuclear phenomenon that I have described seems to be one of
division. Perhaps it is somehow associated with the giving off of the
secretion of the cells, for these nuclei seem to be found in greatest
abundance in those cells in which the secretion is most abundant. In
Conant’s sections I found but little evidence of these nuclear phenomena
as also little secretion, which all goes to show the association of
the nuclear phenomenon with the secretion. I have failed to find any
descriptions in the literature of nuclei to which I could refer my
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