all ordinary needs. For the purpose of microscopic measurements an
ocular micrometer is necessary. This may be obtained as a separate
eye-piece, or as a round piece of glass with measured divisions
marked upon it that may be put into an ocular. The value of the scale
must be determined for every lens and tube-length by estimating the
number of its parts covering one part of a stage-micrometer marked in
hundredths of a millimetre.
[Illustration: FIG. 50.—A satisfactory outfit for the working
laboratory. Continental stand, medium-sized, with large stage, three
objectives, etc.]
An instrument of any one of the above-mentioned makes, carefully
selected and tested, should last its owner a life-time if proper
care is taken of it. It should receive the same careful attention
accorded a good violin or piano. It should be protected from dust,
action of chemicals, heat, sunlight, and rough usage. When carried
it should be supported in such a way that its weight is not thrown
upon the thread of the adjusting screws. The adjustment, draw-tube
and iris diaphragm should be carefully oiled at intervals, using the
least possible amount of the best microtome oil. It is not necessary
here to enter into the construction and theory of the microscope,
as this knowledge has usually been obtained before the pathologic
laboratory is reached. Experience has shown me, however, that it is
always necessary to remind students, even those experienced for some
time in the use of the microscope, of certain fundamental principles
in the adaptation of microscopic technique to pathologic work. The
following rules are of value:—
1. Use a _low-power_ objective for all work except for the study of
bacteria, microparasites and finer cell-structures. The aim should
be to obtain as much of a bird’s-eye view of the “geography” of
the section as possible. Contrast plays a very important part in
pathologic diagnosis; and it is lost in high-power work, so far as
the relations of cells and tissue-elements and pathologic products
are concerned. The student almost invariably enters the pathologic
laboratory with a fixed “high-power habit,” and he is usually greatly
surprised to learn how much he misses with the high-power and how
much he can see with the low-power. A motto used many years in my
laboratory, “Low-power objective and high-power cortex,” is of
greater educational value than may appear at first sight. A slide
should be examined first with the naked-eye, as it is held against
a window or light; then it should be examined under the low power.
Rarely will it be necessary to use a high-power except for the
purposes mentioned above.
2. Weak eye-pieces should be used; strong ones darken the field
and tire the eyes. Only in the case of apochromatic lenses and
compensation oculars can the strong ones be used without darkening
the field too much.
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