For the rapid staining of sections of fresh tissue cut by freezing a section is floated from the salt-solution onto a slide, which is then carefully lifted from the salt-solution and the excess of the latter removed. Several drops of methylene blue, carbol-thionin or carbol-kresyl-echt-violett are run upon the section with the glass-dropper, and allowed to remain for 15-30 seconds. The stain is then washed off with salt-solution, a cover-slip put on and the section examined in the salt-solution. Thionin has been especially recommended (Wood, Strouse and others) for the rapid staining of frozen sections; but I prefer to use carbol-kresyl-echt-violett (kresyl-echt-violett 1 grm., 5 per cent aqueous solution of phenol 80 cc., 95 per cent alcohol 20 cc.). This gives a very good differentiation in the section examined in water, and the picture is clearer than with thionin; the specific staining-reactions with mucin, amyloid, mast-cells, etc., are also more marked than with the latter stain. Such sections are not as clear as dehydrated and cleared sections and their possibilities of diagnosis are correspondingly limited, even in the hands of an expert with sections of this kind. To make permanent balsam-mounts of the frozen unfixed sections the latter must be fixed in formol or alcohol, or by heat (hot water). The sections may be placed in 4 per cent formol for several minutes, then into 80 per cent alcohol, then stained, washed, dehydrated in absolute alcohol, cleared in carbol-xylol and finally mounted in balsam. Hæmatoxylin and eosin may be used for the staining. Fresh sections may also be fixed in hot water, stained in hæmatoxylin, dehydrated in alcohol and cleared in xylol. To save time the sections may be fixed on the slide for a few minutes in alcohol, care being taken to prevent the sections from rolling up by dropping the alcohol onto the middle of the section after it has been carefully flattened out on the slide. The section may then be stained with hæmatoxylin, borax-carmine or other stains, dehydrated, cleared and mounted in balsam. After fixing with alcohol on the slide the section may be attached to the slide by blotting it with absorbent paper, then covering section with absolute alcohol, draining this off after a few seconds and then running over the section a thin solution of celloidin, which is allowed to drain off, leaving a very thin film over the section and slide. The latter is then immersed in water for a few seconds, and the celloidin-film on setting holds the section to the slide, provided the celloidin has been of proper consistence. The section can now be stained, washed, dehydrated, cleared in origanum and mounted in balsam. When mounted the thin film of celloidin is invisible (Wright’s method).