The cover-slips must be handled with forceps and
the section side should always be uppermost. Slides covered with
a film of albumin-glycerin may be used instead of cover-slips.
The albumin-glycerin film may be omitted, and the sections with
glossy side down floated in warm water on to clean covers or
slides; the water is drained off and the slides or covers are put
in the incubator for 12 hours. Sections adhere fairly well by this
method (_capillary attraction method_). Bubbles are removed by
careful heating. Serial ribbons of the size desired can be floated
and mounted on slides by the albumin-glycerin or the capillary
attraction method.
By far the best method of preparing paraffin sections for staining
is the _molasses plate method_, a modification, originating in
my laboratory, of the _Schmorl-Obregia_ sugar-dextrin method.
When many sections are to be stained at once it is the most
convenient method and gives uniform results. In the preparation
of sections for class-work it has no equal. It can be used also
for giving out unstained sections. When many sections must be
stained in diagnostic work the method saves much time and labor.
Fifty sections can be stained as easily as one. It combines all
the advantages of the celloidin and paraffin methods, as does the
_Schmorl-Obregia_ sugar-dextrin method, but is much cheaper than
the latter.
_Schmorl_ advised the use of a sugar-dextrin solution (cane sugar
solution [1:1] 300 cc., 80 per cent alcohol 200 cc., yellow
dextrin solution [1:1] 100 cc.) to be run over a perfectly clean
glass plate or slide until the entire surface is covered with an
even layer. The paraffin sections as they are cut are arranged in
order on the wet plate, and when the plate is full, it is heated
sufficiently to flatten and smooth the sections. The plate is then
placed in an incubator for 3-12 hours to harden and dry. When dry
it is immersed in xylol to take out the paraffin, then treated
with absolute alcohol for 10-15 minutes, the alcohol drained off,
and the plate covered with a thin layer of celloidin (celloidin or
photoxylin 10, absolute alcohol 100, ether 100). As soon as the
celloidin sets (1-2 minutes) the plate is immersed in warm water
and the celloidin film containing the sections is detached. It
can now be carried through the staining, washing, dehydrating and
clearing solutions as one section, and in the clearing solution cut
into strips or single sections, as desired, for mounting. _Huber
and Snow_ improved the method greatly by floating the paraffin
sections directly on to warm dilute sugar-dextrin (a 10 per cent
solution of Schmorl’s stock-solution will suffice), and plating
the sections directly from the latter. This method of using the
dilute solution is less expensive, much cleaner, and saves time
in drying in the incubator. The results are in every way better
than with the Schmorl solution in full strength.
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