The formation of
bubbles and crystals is almost wholly prevented, and less dust is
caught on the plate. In my laboratory we have modified the method
still further by using a 10 per cent solution of New Orleans black
(or baking) molasses instead of the more expensive sugar-dextrin
solution. As the molasses costs but 20 cents a gallon, a gallon of
the dilute solution costing 2 cents can be used indefinitely if
fermentation be prevented by a crystal of phenol or thymol. The
paraffin sections are floated on to this dilute molasses solution
warmed sufficiently to smooth out the sections; 4 × 5 glass plates
(old negatives) thoroughly cleaned and kept in alcohol are immersed
in the warm molasses solution and the sections arranged on them as
desired, lifting out of the solution that part of the plate covered
with sections as they are drawn upon it. As soon as the plate is
covered it is drained, and is then flooded with absolute alcohol.
After 1-2 minutes the alcohol is drained off and the plate flooded
with thin celloidin, which is allowed to set for a minute or so,
and the plate then immersed in warm water in which the celloidin
film containing the paraffin sections is detached. This film is
then handled by catching it at the two corners of one end with
the fingers, or better still by a pair of forceps held in each
hand. The film is put first into xylol to remove the paraffin,
then into 95 per cent alcohol, then into water and thence into the
staining solution. After staining the film is washed, dehydrated
and cleared, and in the clearing solution is cut into strips or
single sections by means of the wheel-shaped paper-cutter used by
paper-hangers. The pieces are then mounted. A dilute sugar-dextrin
solution can be used instead of the molasses-solution, but the
latter is much cheaper and does just as well. Aside from this
advantage our method of transferring the paraffin-sections into the
celloidin film without first removing the paraffin saves a great
deal of time, as it is not necessary to wait for the plates to dry
in the incubator. The same method can be applied to the staining
of single paraffin sections on the slide. The conversion of the
paraffin section into a celloidin preparation without any loss of
time for drying is so quickly and easily carried out that I advise
it above all others. The same method may also be applied to the
staining of fresh and fixed tissues cut on the freezing microtome
or sectioned without imbedding. The success of the plate-method
will depend largely upon the state of the glass-plates when put
into the molasses solution. They must be perfectly clean or the
celloidin sheet will not separate well. It is best to keep them
in alcohol until they are needed. The celloidin must be of the
right consistency, the layer must be thin, and cover the entire
plate uniformly. It must not be allowed to harden too much before
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