_Preparation._ Boil in a porcelain dish resorcin 4 grms., fuchsin (Grübler) 2 grms., and water 200 cc. After the mixture has boiled a few seconds add 25 cc. of liquor-ferri sesquichlor., Pharm. Germ. III. Stir well and boil for 5 minutes. When cool, filter. Carefully loosen the filter from the funnel, transfer it to the same porcelain dish which still contains a small amount of sediment, and add 200 cc. of 94 per cent alcohol. Boil and stir carefully. Remove the filter-paper when all the sediment is dissolved. Cool, filter; make up the filtrate to 200 cc. with 94 per cent alcohol, and to these 200 cc. add 8 cc. of hydrochloric acid. Resorcin-fuchsin may be obtained from Grübler, but the freshly-prepared stain gives better results. 1. Fix in any ordinary solution; imbed; cut. 2. Stain with lithium-carmine and differentiate in acid alcohol; wash thoroughly. 3. Stain in the resorcin-fuchsin mixture for 20-60 minutes. 4. Wash rapidly in acid alcohol. 5. Dehydrate and differentiate in absolute alcohol until section is red. 6. Clear in xylol; balsam. Nuclei are red; elastic fibres blue-black. Should the stain when old give a diffuse staining differentiate for a longer time in acid alcohol. _c._ =Fat Tissue.= Use same methods as advised for the demonstration of fatty degeneration and infiltration (osmic acid, scharlach R, sudan III). V. EAR. Remove temporal bone; fix in formol-Müller’s; decalcify in trichloracetic acid; wash; after-harden in alcohol; imbed in celloidin. For nerve-endings use Golgi’s and methylene-blue methods. VI. EYE. Fix in Müller’s, formol-Müller’s, Zenker’s, formol, Flemming’s or Marchi’s solution. Aid fixation by incisions into sclera. The eye should not be left in formol for more than 3 days. Section as desired; imbed larger pieces in celloidin, small ones in paraffin. Use alum-carmine, iron-hæmatoxylin, Van Gieson’s, Weigert’s elastic stain, Levaditi’s silver-method, Golgi’s nerve-stain, methylene-blue method, etc., according to the object of the investigation. VII. LIVER. For the demonstration of the bile-capillaries _Weigert’s neuroglia method_ gives the best results. (See Page 300.) This method may be used with sections cut on the freezing-microtome after fixation in formol. Such sections are placed in a ½ per cent solution of chromic acid for 1 hour, transferred to the neuroglia mordant for 5-6 hours, washed well with water, and then treated as for the neuroglia method. Van Gieson’s method may also be used for frozen sections of formol-fixed tissue. The walls of the capillaries show as fuchsin-colored streaks. VIII. MUSCLE.