The chief object of a strictly agricultural examination of a field
or garden product is to determine its food value. This purpose can
be accomplished without entering into a minute separation of nearly
allied bodies. For example, in the case of carbohydrates it will be
sufficient as a rule, to separate them into four classes. In the first
class will be found those soluble in water as the ordinary sugars. In
the second group will be found those which, while not easily soluble in
water, are readily rendered so by treatment with certain ferments or by
hydrolysis with an acid. The starches are types of this class. In the
third place are found those bodies which resist the usual processes of
hydrolysis either with an acid or alkali, and therefore remain in the
residue as fiber. Cellulose is a type of these bodies. In the fourth
class are included those bodies which on hydrolysis with an acid yield
furfurol on distillation, and therefore belong to the type containing
five atoms of carbon or some multiple thereof in their molecule. For
ordinary agricultural purpose the separation is not even as complete as
is represented above.
What is true of the carbohydrates applies equally well to the fats and
to other groups. Especially in the analysis of cereals and of cattle
foods, the treatment with solvents is confined to the use in successive
order of ether or petroleum, alcohol, dilute acids, and alkalies, the
latter at a boiling temperature. The general method of treatment with
these solvents will be the subject of the following paragraphs.
=34. Extraction of the Fats and Oils.=—Two solvents are in general use
for the extraction of fats and oils; _viz._, ethylic ether and a light
petroleum. The former is the more common reagent. Before use it should
be made as pure as possible by washing first with water, afterwards
removing the water by lime or calcium chlorid, and then completing
the drying by treatment with metallic sodium. The petroleum spirit
used should be purified by several fractional distillations until it
has nearly a constant boiling-point of from 45° to 50°. The detailed
methods of preparing these reagents will be given in another place. For
rigid scientific determinations the petroleum is to be preferred to the
ether. It is equally as good a solvent for fats and oils and is almost
inert in respect to other vegetable constituents. Ether, on the other
hand, dissolves chlorophyll and its partial oxidation products, resins,
alkaloids and the like. The extract obtained by ether is therefore less
likely to be a pure fat than that secured by petroleum. For purposes of
comparison, however, the ether should be employed, inasmuch as it has
been used almost exclusively in analytical operations in the past.
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