Lead subacetate forms a chemical union with levulose and the resulting
compound has a different rotatory power from the left-handed sugar in
an uncombined state. By adding a sufficient quantity of subacetate
solution, the left-handed rotation of levulose may be greatly
diminished if not entirely destroyed. In this case the dextrose, which
with levulose forms inverted sugar, serves to increase the apparent
right rotation due to the sucrose in solution. The reading of the
scale is therefore higher than would be given by the sucrose alone.
If the lead subacetate could be added in just the proportion to make
the invert sugar neutral to polarized light, its use would render the
analysis more accurate; but such a case could only arise accidentally.
To correct the error, after clarification, the compound of levulose and
lead may be decomposed by the addition of acetic acid according to the
method of Spencer. In this case the true content of sucrose can only be
obtained by the method of inversion proposed by Clerget, which will be
described in another paragraph.
=88. Clarification with Mercuric Compounds.=—Where the disturbing
bodies in a solution are chiefly of an albuminoid nature, one of the
best methods of securing clarification is by the use of a solution
containing an acid mercuric compound.[52] In the case of milk this
method is to be preferred to all others. Albuminoid bodies themselves,
have the property of deflecting the plane of polarization, as a rule,
to the left, and therefore, should be completely removed from solutions
containing right-handed sugars such as lactose. For this purpose the
mercuric compound is more efficient than any other. It is prepared and
used as follows.[53] Dissolve mercury in double its weight of strong
nitric acid and dilute the solution with an equal volume of water. One
cubic centimeter of this solution is sufficient to clarify fifty times
its volume of milk.
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