=89. Decolorization by Means of Bone-Black.=—Where the means already
described fail to make a solution sufficiently colorless to permit of
the passage of a ray of polarized light, recourse should be had to a
decolorizing agent. The most efficient of these is bone-black. For
laboratory work it is finely ground and should be dry if added to an
already measured solution. When moist it should be added to the flask
before the volume is completed, and a correction made for the volume of
the dry char employed. Bone-black has the power of absorbing a certain
quantity of sugar, and for this reason as little of it should be
employed as is sufficient to secure the end in view. If not more than
one gram of the char be used for 100 cubic centimeters of solution,
the error is not important commercially. The error may be avoided by
placing the char on the filter and rejecting the first half of the
filtered solution. The char becomes saturated with the first portion
of the solution, and does not absorb any sugar from the second. This
method, however, does not secure so complete a decolorization as is
effected by adding the black directly to the solution and allowing to
stand for some time with frequent shaking.
=90. Remarks on Analytical Process.=—Since large weights of sugar are
taken for polarization, a balance which will weigh accurately to one
milligram may be used. In commercial work the weighing is made in a
counterpoised dish with a prominent lip, by means of which the sample
can be directed into the mouth of the flask after partial solution.
Where the air in the working room is still, an uncovered balance is
most convenient. With a little practice the analyst will be able to
dissolve and transfer the sample from the dish to the flask without
danger of loss. The source of light used in polarizing should be in
another room, and admitted by a circular opening in the partition. In a
close polarizing room, which results from the darkening of the windows,
the temperature will rapidly rise if a lamp be present, endangering
notably the accuracy of the work, and also interfering with the comfort
of the observer. The greatest neatness must be practiced in all stages
of the work, and especially the trough of the polariscope must be kept
from injury which may arise from the leaking of the observation tubes.
Dust and dirt of all kinds must be carefully excluded from the lenses,
prisms, wedges and plates of the instrument.
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