=101. Specific Rotatory Power.=—In order to compare among themselves
the rotations produced on a plane of polarized light by different
optically active bodies in solution, it is convenient to refer them all
to an assumed standard. The degree of rotation which the body would
show in this condition, is found by calculation, since, in reality,
the conditions assumed are never found in practice. In the case of
sugars and other optically active bodies, the standard of comparison
is called the specific rotatory power. This factor in any given case,
is the angular rotation which would be produced by any given substance
in a pure anhydrous state if it were one decimeter in length and of a
specific gravity equal to water. These are conditions which evidently
do not exist in the case of sugars, since crystalline sugar particles
have no polarizing power, and it would be impossible to pass a ray
of light through an amorphous sugar column of the length specified.
The specific rotatory power is therefore to be regarded as a purely
theoretical factor, calculated from the actual data obtained by the
examination of the solution of any given substance. If the length
of the observation tube in decimeters be represented by _l_, the
percentage of the polarizing body in 100 grams by _p_, and the specific
gravity of the solution by _d_, and the observed angle of rotation by
_a_, then the factor is calculated from the formula:
_a_. 100
[_a_]_{Dj} = ---------------.
_p_. _d_. _l_.
The symbols Dj refer to the character of light employed, D indicating
the monochromatic sodium flame, and j the transition tint from white
light.
If the weight of the polarizing body _c_ be given or known for 100
cubic centimeters of the solution the formula becomes
_a_. 100
[_a_]_{Dj} = ----------.
_c_. _l_.
The latter formula is the one easier of application since it is only
necessary in applying it to dissolve a given weight of the active body
in an appropriate solvent and to complete the volume of the solution
exactly to 100 cubic centimeters. It is therefore unnecessary in this
case to determine the specific gravity.
Public-domain text, read in full here on John Shaqi.
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