Research methods in ecologyClements, Frederic E. (Frederic Edward)
Science
Research methods in ecology
Clements, Frederic E. (Frederic Edward)
Plant ecology; Plant ecology -- Methodology
=84. Kinds of standards.= The base standard is the one taken at Lincoln
(latitude 41° N.) at meridian June 20–22. This is properly the unit to
which all exposures are referred, but it has been found convenient to
employ the Minnehaha standard as the base for the Colorado mountains, in
order to avoid reducing each time. _Relative_ standards are frequently
used for temporary purposes. Thus, in comparing the light intensities of
a series of formations, one to five standards are exposed on the solio
strip before beginning the series of readings. _Proof_ standards are the
exposed solio strips, which fade in the light, and can, in consequence,
be kept only a few weeks without possibility of error. The fading can be
prevented by “toning” the strip, but in this event the exposures must be
fixed in like manner before they can be compared. This process is
inconvenient and time-consuming. It is also open to considerable error,
as the time of treatment, strength of solution, etc., must be exactly
equivalent in all instances. _Permanent_ standards are accurate
water-color copies of the originals obtained by the photometer. These
have the apparent disadvantage of requiring a double comparison or
matching, but after a little practice it is possible to reproduce the
solio tints so that the copy is practically indistinguishable from the
original. The most satisfactory method is to make a long stroke of color
on a pure white paper, since a broad wash is not quite homogeneous, and
then to reject such parts of the stroke as do not match exactly.
Permanent standards fade after a few month’s use, and must be replaced
by parts of the original stroke. _Single_ standards are made by one
exposure, while _multiple_ ones have a series of exposures filling a
whole light strip. These are regularly obtained by making the exposures
from 1–10 seconds respectively, and then increasing the length of each
successive exposure by 2 seconds. Single exposures of 1–5 seconds as
desired usually serve as the basis for permanent standards, but a
multiple standard may also be copied in permanent form. Exposures for
securing standards must be made only under the most favorable
conditions, and the length in seconds must be exact. The use of the
stop-watch is imperative, except where access may be had to an
astronomical clock with a large second hand, which is even more
satisfactory. The length of time necessary for the series desired is
reckoned beforehand, and the exposures begun so that the meridian falls
in the middle of the process.
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