In sexually differentiated individuals, the difference of sex is already apparent in the embryonal state of existence, not only in the exterior form, but also in the interior cellular rudiments which subsequently form the genital organs. In both, the earliest forms are of such a nature that, up to a certain period, it is impossible for investigations conducted with the means at present at our disposal to discover any distinction. Soon, however, after this, in such organisms as have a distinction of sex, elementary male and female forms of the organs of reproduction can be recognized developing themselves in the embryo out of the substratum of formative elements. Some of these remain in a rudimentary condition; others attain to complete development. These processes take place at a relatively early period. They do not seem to make their first appearance, as phenomena of vitality, in the course of the life-development of the cells of the ovum. But it is not improbable that, from the very outset, the ovule has a capacity to transfer (during the process of segmentation) to a corresponding cell-substance (out of which the generative organs will be subsequently developed) the force contained in the ovule, so that the cell-substance may afterwards take up the office of providing for the preservation of the species. The cells of the ovum derive this power from the protoplasma of the ovum, and retain it in a rudimentary form for one sex, whilst for the other they possess it in full measure. This energy is contained in the ovule itself in an unknown condition. In it lies the basis of the formation and development of the future sex. In close connection with this property of the ovule, lies another faculty, included in the ovule, namely, that the other different elements proceeding from the cell-body of the ovum, starting from the protoplasma of the ovum, are endowed with certain vital peculiarities, according as they belong to the future male, or female, organisms. It will be plain from this that the germ of the future sex must be sought in the first cell-segmentation of an ovum. As soon as some of the cells derived from the primary protoplasm of the ovum have developed themselves into genital cells, the other elements which have originated from the same ovum are in such a manner conditioned that, in the latter stages of their vitality, they adapt themselves, and, in short, adapt the properties inherent in all the cells, to the sex of the individual. According, as the ovum is male or female, so are also the cells which originate from it either all male or all female.