It is well at the beginning of the procedure to put a few drops of formaline into the measuring-glass, so that the urine may not, in consequence of standing, decompose, and so become unfit for accurate analysis. Of the collected quantity of twenty-four hours, about 200 grammes should be poured into a small phial, well corked, and used for analysis. In making the analysis it is best to proceed in the following order: First, we determine the _reaction_ of the urine with litmus paper. In normal urine the reaction is generally acid. Next the _specific gravity_ is determined. This is most easily done with Ultzmann’s urometer, by means of which the density of the urine can be easily determined. That varies in normal urine generally between 1015 and 1020. In exceptional cases it may sink very low, which often happens after much fluid has been taken. In other cases it rises under pathological circumstances enormously high, as, for example, in diabetes. In the case of a thorough preparation of the organism by the use of a great quantity of concentrated nitrogenous food with a view to influencing sex, the specific gravity very often reaches 1030 and more. After the specific gravity we measure next the _quantity of urine_ collected in the twenty-four hours. * * * * * We proceed next to determine the normal _urine-sugar_, and for this purpose use a number of the well-known tests--Nylander’s, the fermentation test, and Trommer’s test--which have been already described. If these give a positive result, we proceed to a quantitative examination by means of the polariscope. If the quantity of sugar found is very small, we exert ourselves to get rid of it by a suitable diet, because otherwise no certain influence over the embryo in the direction of the production of male offspring can be exercised. If, however, we find no sugar by any of the above tests, we seek for it by means of the phenylhydrazin test also described above. A few experiments on the melting point of the phenyl-glycosazon crystals will easily give us certain information. In the analysis we observe particularly whether the positive result of the phenylhydrazin test has originated from the sugar or from the reducing substances. With the polarization apparatus we determine the quantity of lævo-rotatory substances, _the optical rotation_, in per cents., as these stand in a certain relation to the reducing substances. We make these experiments with urine that has not been decolorized. The former becomes greater as the quantity of the latter increases. * * * * *