Depre[3] determines fusel oil by oxidizing a definite quantity of the alcohol in a closed vessel with potassium bichromate and sulphuric acid. after removal of excess of the oxidizing reagents, the organic acids are distilled, and, by repeated fractional distillation, the acetic acid is separated as completely as possible. The remaining acids are saturated with barium hydroxide, and the salts analyzed; a difference between the percentage of barium found and that of barium in barium acetate proves the presence of fusel oil, and the amount of difference gives some idea of its quantity. Betelli[4] dilutes 5 c.c. of the alcohol to be tested with 6 to 7 volumes of water, and adds 15 to 20 drops of chloroform and shakes thoroughly. If fusel oil is present, its odor may be detected by evaporating the chloroform; or, by treatment with sulphuric acid and sodium acetate, the ether is obtained, which can be readily recognized. Jorissen[5] tests for fusel oil by adding 10 drops of colorless aniline and 2 to 3 drops of hydrochloric acid to 10 c.c. of the alcohol. In the presence of fusel oil a red color is produced within a short time, which can be detected when not more than 0.1 per cent. is present. But Foerster[6] objects to this method because he finds the color to be due to the presence of furfurol, and that pure amyl alcohol gives no color with aniline and hydrochloric acid. [Footnote 3: Pharm. J. Trans. [3] vi., 867.] [Footnote 4: Berichte d. Deutschen Chem. Gesellsch., viii., 72.] [Footnote 5: Pharm. Centralhalle, xxii., 3.] [Footnote 6: Berichte d. Deutsch. Chem. Gesellsch., xv., 230.] Hager[7] detects fusel oil as follows: If the spirit contains more than 60 per cent. of alcohol, it is diluted with an equal volume of water and some glycerine added, pieces of filter paper are then saturated with the liquid and exposed to the After the evaporation of the alcohol, the odor of the fusel oil can be readily detected. For the quantitative determination he distills 100 c.c. of the alcohol in a flask of 150 to 200 c.c. capacity connected with a condenser, and so arranged that the apparatus does not extend more than 20 cm. above the water bath. This arrangement prevents the fusel oil from passing over. If the alcohol is stronger than 70 per cent., and the height of the distillation apparatus is not more than 17 cm., the residue in the flask may be weighed as fusel oil. With a weaker alcohol, or an apparatus which projects further out of the water bath, the residual fusel oil is mixed with water. It can, however, be separated by adding strong alcohol and redistilling, or by treating with ether, which dissolves the amyl alcohol, and distilling, the temperature being raised finally to 60 deg.. [Footnote 7: Pharm. Centralhalle, xxii., 236.]