Scientific American Supplement, No. 492, June 6, 1885Various
Science
Scientific American Supplement, No. 492, June 6, 1885
Various
Science -- Periodicals
Farrant's solution may be substituted for glycerine in many instances,
because of its feebler tendency to render the tissues transparent. It
consists of equal parts of gum arabic, glycerine, and a saturated
solution of arsenious acid. In mounting preparations with this medium,
the covered object should be allowed to lie a day before the varnish is
applied, so that the cover may be fixed, and thereby prevented from being
displaced. Rectified spirits may be used for mounting softened bone and
tooth, and naphtha and creosote are useful for preserving urinary casts.
When the section is mounted in Canada or Dammar balsam, no cement is
required, but for all other preservative media the margin of the cover
must be covered with cement. To do this, dry the edges of the cover
thoroughly with bibulous paper, and paint a layer of gold size, allowing
it to overlap the cover an eighth or sixteenth of an inch, then cover
this with white zinc cement.
_Preparation for mounting the different tissues_.--To obtain a section of
bone or tooth requires a grinding down of the tissue until it is so thin
as to be transparent. A section should first be cut as thin as possible
by a fine saw. It should be attached by the flattest side to a piece of
glass, and then ground down by a grindstone or by very fine emery, on a
perfectly flat piece of lead. When sufficiently thin and transparent,
mount in rectified spirits or Dammar. Sections of the tongue may be made
by embedding in paraffin, and mounted in Farrant's solution or glycerine.
Sections of the stomach may also be made by embedding in paraffin, but
better ones can be made by freezing. Farrant's solution makes a good
mounting.
The intestines also give a better section from freezing than by
embedding, as the paraffin injures the villi; mount in the same medium as
the stomach.
The liver may be embedded in paraffin, and the section mounted in
Farrant's solution or glycerine. The kidney may be treated in the same
way. The cornea of the eye can be readily cut by embedding in paraffin,
and the section may be mounted in Farrant's solution. The crystalline
lens and retina may be treated similarly.
The brain and spinal cord should be embedded in paraffin or a carrot, and
the section mounted in Dammar. Sections of the uterus and ovaries are
best mounted in glycerine or Dammar. Sections of lung maybe made by
embedding in gum or by freezing, and mounted in Farrant's solution.
Every slide should be of uniform size, and labeled. The usual size is 3×1
inches, and should be of a good quality of glass, free from scratches or
air holes. They may be labeled either by writing with a diamond, or a
small piece of paper affixed to one end, on which is written what is
required.
* * * * *
LIFE HISTORY OF A NEW SEPTIC ORGANISM.
Public-domain text, read in full here on John Shaqi.
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