As, in nearly all of the methods which were employed for estimating carbonic acid in the air, provision is not made for the exclusion of air not measured containing carbonic acid from the alkaline fluid before titrating or weighing, the results are generally too high and show a far greater variation than is found by more exact methods. For example, Gilm[10] found from 36 to 48 volumes; Levy's[11] average is 34 volumes; De Luna's[12] 50 volumes; and Fodor's,[13] 38.9 volumes. Admitting that the quantity of carbonic acid in the air is subject to variation, yet the results of Reiset's and Schultze's estimations go to prove that the variation is within narrow limits. [Footnote 10: Sitzungsher. d. Wien. Akad. d. Wissenschaften, 34, 257.] [Footnote 11: Ann. d. l'Observ. d. Mountsouris, 1878 and 1879.] [Footnote 12: Estudios quimicos sobre el aire atmosferico, Madrid, 1860.] [Footnote 13: Hygien. Untersuch., 1, 10.] Indiana University Chemical Laboratory, Bloomington, Indiana. --_Amer. Chem. Journal._ * * * * * ANALYSIS OF KOLA NUT. Alkaloids or crystallizable principles: Per Cent. Caffeine. 2.710 Theobromine. 0.084 Bitter principle. 0.018 Total alkaloids. ----- 2.812 Fatty matters: Saponifiable fat or oil. 0.734 Essential oil. 0.081 Total oils. ----- 0.815 Resinoid matter (_sol. in abs. alcohol_) 1.012 Sugar: Glucose (_reduces alkaline cuprammonium_). 3.312 Sucrose? (_red. alk. cupram. after inversion_)[1]. 0.602 Total sugars. ----- 3.914 Starch, gum, etc.: Gum (_soluble in H2O at 90 deg. F_.). 4.876 Starch. 28.990 Amidinous matter (_coloring with iodine_). 2.130 Total gum and fecula. ----- 35.999 Albuminoid matters. 8.642 Red and other coloring matters. 3.670 Kolatannic acids. 1.204