Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
8. _Microtome._—Although the preceding plans may be sufficient to answer
all his requirements, if the student wishes to obtain only one or two
sections of small dimensions, of a given object, if he requires a number
of such sections he will find these methods fail him, for even though by
practice he may have attained to considerable aptitude in the use of the
knife, it will still unquestionably happen that the vast majority of his
sections will be more or less imperfect. If, therefore, it be desired to
procure a number of perfect sections, of equable thickness and large
area, it is absolutely necessary to resort to the use of some form or
other of microtome, or section-cutter. This instrument, in its simplest
form, merely consists of a stout brass tube closed at one end, and being
by the other fixed at right angles into a smooth plate of metal. A plug
or disk of brass, accurately fitting the interior of the tube, is acted
upon by a fine threaded screw piercing the base of the tube, and by
means of which the plug, and any object it may support, can be elevated
at pleasure. The object by this means being made gradually to rise out
of the tube, sections are cut from it by simply gliding a sharp knife
along the smooth cutting-plate, and hence across the specimen. Any
intelligent worker in brass would make an instrument of this kind at a
very small cost, and although perhaps it might lack the finish of an
instrument bought at the optician’s, it would, if accurately made, do
its work as well as the most complicated and expensive. If, however, the
student resolves to purchase a microtome, there are a variety of forms
in the market from which he may choose. A few hints may perhaps be of
service in enabling him to make a judicious selection. At the outset we
may say that unless the student intends to devote himself solely to the
production of sections of wood, etc., he ought not to procure one of
those forms of microtome known as wood section-cutters, in which the
object to be cut is held in position in the tube by means of a binding
screw which pierces its side.[4] Although these machines are all that
can be desired for cutting hard bodies, they are not so suitable for
soft ones. The chief points to be attended to in selecting a microtome
are, (1) that the cutting-plate of the instrument be made of glass, or
in default of this, of very hard metal of the most perfect
smoothness;[5] (2) that the diameter of the tube be neither too large
nor too small—it ought not to be less than 5/8-in., or greater than 1
inch;[6] (3) that the screw, which should be _fine_ and well cut, be
provided with a graduated head; (4) that there be some kind of index by
which fractional portions of a revolution of the screw may be measured;
and (5) that the plug fit the tube of the microtome so accurately that
when melted paraffine, gum, or other imbedding agent be poured into it,
it may not find its way between the plug and side of the tube (§ 18). It
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