Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
10. _Section-Knife._—Of not less importance than the microtome is the
section-knife, to be used in conjunction with it. How perfect soever the
former, and whatever the dexterity of the operator, unless he be
provided with a suitable and well-made knife, he will never succeed in
obtaining satisfactory results. As to the most desirable _size_ of the
knife, much difference of opinion seems to exist, section knives varying
in this respect from a blade of extreme shortness to one which fell
under our observation, in which the portentous length of _thirteen_
inches was attained. What advantages were to be expected by prolonging
the blade to this extravagant length, must remain an inscrutable mystery
to all save its designer. Concerning the _shape_ of the knife, it is
frequently advised that the surface which has to glide along the
cutting-plate of the microtome should be ground _flat_. A most
unsuitable arrangement, as a very little actual experience of
section-cutting will speedily demonstrate. After many unsuccessful
attempts to obtain a really good and reliable section-knife, we
determined to have one specially made, which, as it has proved
everything that could be desired, merits a brief description. It is of
the utmost importance that the blade be made of good and well-tempered
steel, not only that it may be capable of receiving an edge of the most
exquisite keenness, but also that it may _retain it_. The knife of which
we speak (and which was made by Mr. Gardner, of South Bridge, Edinburgh)
is furnished with a blade _four inches_ long, and 7/8-inch broad, set
into a square handle of boxwood, also four inches in length. The
thickness of the blade at the back is not quite 1/4-inch, while _both_
of its surfaces are slightly hollow ground. It is essentially necessary
that the back and edge of the blade be strictly parallel to each other,
otherwise the knife, when in use, will have such a tendency to tilt over
as to render its management extremely difficult. It is very easy to
discover if this condition be fulfilled, for if on carefully laying the
flat of the blade upon a piece of level glass, every portion of both
back and edge are found to be in close contact with it, the knife may in
this respect be considered perfect. Every student who aspires to be a
successful section-cutter should provide himself with a good Turkey
oilstone, _and learn to use it_. He should also possess a razor strop,
as it will be in constant requisition.[7] It may here be remarked that
though _razors_, as a rule, are unsuitable for use with the microtome
from want of uniformity in the thickness of their blades, yet, if only a
small object is to be cut—for instance, a thin root or stem—very good
results may be obtained from their use, especially if one of the
old-fashioned make, having a thick back and slightly _concave_ surfaces,
be employed.
Public-domain text, read in full here on John Shaqi.
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