Remove the tissue from the solution on to blotting paper, when the superfluous gum will speedily drain off, and in two or three minutes the _surface_ will have become quite glazy and dry. Having melted some paraffine mixture in the water-bath, the tissue held in the forceps must be plunged for an instant into the heated liquid and immediately withdrawn, when the crust of paraffine with which it is enveloped will promptly harden. Whilst this is taking place we may make ready the microtome. Having by means of the milled head or handle depressed the plug in the tube so as to leave a free opening about an inch deep at its upper end, we must pour in the melted paraffine, which by this time will have become a little cooler, until the cavity be about half filled. The prepared tissue must now be introduced, care being taken to place it in such a position that the sections may be cut in the desired direction. The tissue must, if necessary, be held in position with forceps or a needle point, till the imbedding material becomes hard enough to give it due support. It is here to be remembered that it will not be advisable to place the tissue in the centre of the tube—it will be much more easily cut if placed rather nearer to that edge of the tube which is situated next the operator in the act of cutting. More paraffine is to be slowly added, until the tissue is completely covered; even after this still more should be added, for it will be found that in cooling the paraffine shrinks so as to leave a cup-shaped depression in its centre, whereby portions of the tissue which were previously covered are again laid bare. The best method of preventing this is to use the paraffine at as low a temperature as possible, and to use plenty of it. The microtome, with its contents, must now be removed to a cool place, when the paraffine will soon become solidified. Whilst this is being accomplished we may make our further preparations. The first thing we require will be a large basin, full of freshly-filtered water, and provided with a cover. A small beaker of methylated spirit, with a dipping rod or pipette, will also be necessary. We must now see that the section-knife is in thorough order, to ensure which it will be advisable to give it a few turns on the strop. An ordinary razor will also be of service.