Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
22. _Canada Balsam_, as ordinarily met with, is a thick resinous balm of
great viscidity, but readily rendered perfectly fluid by the application
of heat. Formerly, sections were mounted in this medium in its pure
state, but owing to the annoyance which was so constantly being
experienced from the tenacity with which intruding air-bubbles were held
by the viscous medium, this plan of mounting is rapidly falling out of
use.[14] It is now usual to employ the balsam in a diluted condition,
the two chief diluents being chloroform and benzole. As balsam, however,
often contains more or less moisture, it is desirable to drive this off
before adding the diluent. A very convenient way of doing so is to
expose some pure balsam to the heat of a cool oven for several hours,
when the balsam will be found to have assumed a hard, vitreous
character. It should now be broken into small pieces, these put into a
bottle, and some methylated chloroform added, which in a little while
will completely dissolve the hardened balsam. More chloroform is then to
be added, until a solution is obtained sufficiently thin to run through
filtering-paper. A glass spirit lamp must now be procured, having a
capacity of about two ounces, and provided with a cap. Into the
wick-holder of this (which must be made of porcelain) a hollow glass
tube is to be so fitted that its end dips into the lamp to within about
a third of the bottom. (See Fig.) The thin chloroform-balsam is now to
be filtered into this lamp, very fine filtering-paper—through which a
little chloroform has first been passed—being used for the purpose. When
the lamp is full it must (deprived of its cap) be put in a warm place
until sufficient of the chloroform has evaporated to leave behind it a
fluid of the consistence of thin syrup.
Footnote 14:
Public-domain text, read in full here on John Shaqi.
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