Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
After cutting in gum, the sections are gently removed from the knife
into distilled water by a soft camel’s hair brush, and left there for
an hour or two, until the medium is entirely dissolved out. They may
then be stained and mounted, or they may be put away in spirit for an
indefinite time, and then stained and mounted.
*Celloidin* is for many purposes almost an ideal embedding medium. (1)
It has great penetrating power; (2) it can be made of an admirable
consistence for cutting purposes; (3) after sections are made it allows
them to be very freely manipulated without fear of injuring them: (4)
and being perfectly transparent and homogeneous in thin sections, it
does not require to be removed from a section before mounting. It is
insoluble in water, and in weak spirit; slightly soluble in alcohol of
more than 90 per cent. strength, and very readily soluble in ether, or
in a mixture of alcohol and ether. The last solvent is the one commonly
employed.
The embedding solution is made thus:--
Take some pure celloidin (“Schering’s,” sold in boxes containing an
ounce of shavings, is very good) and pour on it about eight times its
volume of a mixture of equal parts of absolute alcohol and ether. Allow
this to stand all night until the celloidin is dissolved. The solution
should be made about the consistence of ordinary mucilage.
It is also convenient to have a thinner solution made by using double
the proportion of alcohol and ether. Both solutions should be kept in
wide mouthed stoppered bottles, and the stopper should be well greased
with vaseline as an additional obstacle to the evaporation of the
volatile ether.
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