Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
When sections are cut they may be transferred singly to the slide
(which should be lightly smeared beforehand with a saturated solution
of celloidin in oil of cloves), or they may be cut so that the back of
one section of the paraffin block adheres to the front of the next,
and in this way a continuous delicate ribbon of serial sections is
obtained. The ribbon is broken up into lengths of about two and a half
inches and transferred to the slide, on which several ribbons may be
placed side by side, and so a large number of sections kept in the
order in which they are cut. A mark should be made on the slide to
indicate where the series begins, and each slide should be numbered, so
that the exact position of each section in the series can be recognised
at once.
Before mounting, the paraffin must be removed from the sections. This
is easily done on the slide in the case of single sections and of
ribbons. If the sections are curled, a little warmth will make them
unbend and lie flat. The slide is warmed over a spirit lamp until the
paraffin just melts. The sections will keep their places owing to the
celloidin beneath. Xylol is then allowed to flow over the slide from a
pipette, until the paraffin has been completely dissolved, which can
be ascertained by glancing at the sections under the low power of the
microscope. The slide is placed in an almost vertical position to let
the xylol drain off, excess is wiped off from the edge of the slide
with blotting paper, a drop of Canada balsam solution (p. 61) is run on
the slide, and a cover-glass of suitable size is applied.
*Microtomes.*--After a large amount of practice, persons with a fair
amount of manual dexterity may acquire sufficient skill to be able to
cut very satisfactory sections of specimens embedded in paraffin, &c.,
by hand. In the Pathological Laboratory of a large German University,
until quite recently the use of a microtome was prohibited by the
Professor, who is himself a most distinguished histologist. The amount
of time expended before one acquires the necessary skill, and the
cheapness and great convenience of the modern microtome have combined
to throw hand cutting into the background, and some form of microtome
is now almost universally adopted.
[Illustration: Fig. 4.--Cathcart’s Ether Spray Microtome.
A, B. Wooden frame and supports. C. Glass runners. G. Screw for raising
the zinc plate H. J. Ether bottle. L. Tube from air bellows.]
Of these there are a very large number in the market, each having
special advantages, and often special drawbacks. A few of the more
generally useful only will be described. We have microtomes for cutting
in gum frozen by ether spray or ice, and those intended for cutting in
paraffin or celloidin.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account