Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
*The Cambridge Rocking microtome.*--This instrument, as made by the
Cambridge Scientific Instrument Company, or the slightly modified form
made by Messrs. Swift (fig. 8), is the best instrument for cutting
sections of small objects embedded in paraffin. Ribbons of serial
sections can be obtained from it with greater ease and certainty than
with other microtomes. This microtome differs from those which have
been previously described in that the knife is fixed, while the
object is moveable. The microtome consists of an oblong heavy metal
stand. A long bar is arranged so that it rides in see-saw fashion on
two strong vertical pillars arising from the frame. One end of this bar
is hollow, and receives the piece of wood carrying the tissue embedded
in paraffin, which is firmly clamped in position. This end is depressed
by means of a strong spiral spring. In order to raise it there is an
arrangement by which the other end of the bar is depressed by a cord
which revolves round a pulley. When the handle is turned, the tissue
is raised, and when the cord is relaxed, the spring pulls the tissue
firmly and steadily down. The razor, which must have a straight edge,
is fixed firmly by screws, with its edge upwards at the end of the
microtome. The object is then adjusted so that in its descent a thin
slice is taken off by the razor. There is an ingenious arrangement
by which the depression of the bar to raise the section pushes it a
little further in the direction of the razor. The distance can be
graduated from 1/500 to 1/3000 inch. The actual working of the machine
is therefore very simple. The position of the block containing the
tissue to be cut having been adjusted so that the razor just cuts it,
the free end is depressed by means of the pulley. This also pushes the
section a little beyond the razor. The strong spring then draws the
tissue steadily past the edge of the razor, and a thin section is left
on the blade. This may be at once transferred to a slide, or if the
paraffin be of the proper consistence, another cut may be made, when
the two sections should adhere by their edges, and so by repeating the
movement a continuous ribbon may be obtained. If there is difficulty in
obtaining a good ribbon, it will usually be got over by taking a little
soft paraffin and attaching it by means of a hot needle to the lower
end of the paraffin block. The cost of the instrument is about £5.
[Illustration: Fig. 8.--Swift’s Modification of the Cambridge Rocking
Microtome.]
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