Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
*Transitional epithelium.*--Occurs in the pelvis of the kidney, ureter
and bladder. It is very readily detached, especially if not hardened
immediately after death. Remove as early as possible. If the bladder
is taken it should be cut open and pinned out as flat as possible.
Harden in osmic acid, or Müller’s fluid and spirit. Embed preferably in
celloidin.
*Simple columnar epithelium.*--Occurs in many parts. It may be studied
in the salivary ducts, the intestine, kidney, &c., of any mammal.
*Goblet-cells.*--Seen abundantly among the columnar cells of the
intestinal glands, and in the mucous glands of the mouth and of the
cervix uteri.
*Stratified columnar epithelium.*--Occurs only in the urethra. Harden
the penis of a cat in Müller’s fluid, and cut transverse sections.
*Ciliated epithelium.*--Harden the trachea of a recently killed cat in
osmic acid or Müller’s fluid. Beautiful specimens may also be obtained
from an ordinary nasal polypus, which should be put into hardening
fluid immediately after removal.
Stain all sections of epithelium in picrocarmine, and in eosine and
hæmatoxyline.
*Ordinary areolar tissue.*--Difficult to obtain free from fat. It may
be studied in the subcutaneous tissue of the section of the cat’s penis
already made. A fragment of the tissue should also be removed and
carefully teased in a drop of picrocarmine. Areolar tissue may also
be studied in sections of skin, and in the capsules of the different
internal organs.
*Elastic tissue.*--May also be studied in most sections of skin. If
the ligamentum nuchæ of a large quadruped (horse, bullock), &c., is
available it yields the best specimens, or the human ligamenta subflava
may be examined. Pin a piece out on a piece of wood or wax. Harden
in Müller’s fluid. Stain in picrocarmine. Both sections and teased
specimens should be prepared.
*Tendon.*--Readily obtained from an amputated limb. Harden in Müller’s
fluid. Make transverse and longitudinal sections. Stain with eosine and
hæmatoxyline.
A preparation should also be made by teasing a little of the fresh
tendon in normal salt solution, and staining with picrocarmine.
*Retiform* or *lymphadenoid tissue*.--Seen in lymphatic glands and
in the lymphoid follicles scattered along the sub-mucous coat of the
alimentary canal.
Prepare sections in the ordinary way. Stain in eosine and hæmatoxyline
or in picrocarmine.
Some sections should also be prepared by pencilling (_i.e._, dabbing
with a camel’s hair brush) or by shaking sections up in a test tube
with water or normal salt solution. By this means the leucocytes are
removed, and the structure of the adenoid tissue itself becomes more
evident.
*Fat.*--Best studied in sections of skin and subcutaneous tissue, or
in the mesentery of the cat. One specimen should be stained with osmic
acid and picrocarmine and mounted in Farrant’s medium, and another in
eosine and hæmatoxyline and mounted in Canada balsam.
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