Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
*Double or triple nose-piece* (fig. 1).--This mechanical arrangement
is placed on the lower end of the tube. Two or three objectives
of different magnifying power are attached to it. The nose-piece
rotates round a central pivot in such a way that the objectives can
successively be brought accurately into position above the object on
the stage. It is, therefore, a moment’s work to replace a high power
objective by a low power one and _vice versa_. It is an extremely
convenient time-saving appliance, and by its use the risk of dropping
and injuring the objectives when screwing them on and off frequently
is avoided. Those whose microscopes are not already fitted with this
appliance can easily have one fitted on at a cost of about a sovereign.
*Substage condenser.*--This mechanism for concentrating light on the
object is a necessity for bacteriological work. The most convenient
form is *Abbe’s illuminating apparatus* (fig. 2).
This consists of a system of short focus lenses which collects the
light received by the mirror, and throws it on the object. The amount
of light received from the mirror is controlled by an “iris diaphragm,”
the aperture of which can be dilated or contracted by moving a small
lever at the side. It can be fitted on to most microscope stands, but
it is better to get a stand in the first instance which is constructed
to carry one.
The cost of a microscope varies from two guineas to two hundred. There
are many excellent microscopes in the market, and of these several may
be mentioned which the writer has found to work satisfactorily.
[Illustration: Fig. 2.--Abbe’s Illuminating Apparatus.]
Of the cheaper student’s microscopes the “Star” microscope made by
Messrs. R. and J. Beck, of Cornhill, E.C., will be found a safe
investment. It may be obtained with coarse and fine adjustment,
nose-piece, and 1 in. and 1/4 in. objectives, for about £5. Those who
require a better instrument will find Beck’s “Pathological” microscope
fitted with nose-piece, Abbe’s illuminator, &c., for £16, meet all
requirements.
Leitz of Jena, supplies two good and cheap microscopes for £3 10_s._
and £5. They are not, however, of uniform excellence, and they should
be carefully tested by some competent judge before the purchase is
completed. Leitz immersion lenses are cheap, and often extremely good,
but should be carefully tested beforehand, as their quality is not
quite uniform. The microscopes can be obtained from Mr. A. Frazer,
Teviot Place, Edinburgh.
The “Bacteriological” microscope, made by Messrs. Swift, of Tottenham
Court Road, is one with which no one can be disappointed. It is sold
with Abbe’s condenser, triple nose-piece, 1/6 in., and a 1/12 in.
immersion objective, for just under £20. Both stand and lenses are
turned out in Swift’s first-class style, and those who can afford the
initial outlay will not regret it. Or the stand may be purchased, and
the objectives and accessories added singly from time to time.
Public-domain text, read in full here on John Shaqi.
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