Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
Harden in Müller’s fluid. Stain in picrocarmine or eosine and
hæmatoxyline. Also stain sections in safranine, which stains the
colloid material, and also picks out any colloid formation in the cells
themselves.
*Thymus.*--Remove from a fœtus or a very young animal, and prepare in
the usual way.
*Tongue.*--That of the cat or rabbit serves very well.
Ordinary transverse sections should be made, and also sections through
the circumvallate papillæ in order to study the “taste buds.”
*Salivary glands.*--Those of a cat or dog do very well.
Sections should be made from each of the three glands.
*Stomach.*--That of the cat or dog should be studied. The organ must
be removed immediately after death before any post-mortem digestion of
the coats has occurred. The stomach should be opened, washed gently and
pinned out flat, with as little stretching as possible on a piece of
wood, and hardened in Müller’s fluid.
Sections should be made (_a_) longitudinally through the cardiac end to
show the transition from the œsophageal to the gastric mucous membrane,
(_b_) from a portion of the greater curvature, (_c_) longitudinally
through the pyloric valve.
Eosine and hæmatoxyline form the best stain for the alimentary canal.
*Intestine.*--Prepare in the same way as the stomach. Make sections
from (_a_) the upper part of the duodenum to show Brunner’s glands,
(_b_) the ileum, (_c_) a Peyer’s patch, (_d_) the vermiform appendix,
(_e_) the colon.
*Liver.*--Make an injection of one specimen with carmine and gelatin
(p. 120). Harden in methylated spirit. Others should be hardened in
Müller’s fluid and stained in the usual way.
*Kidney, supra-renal, and pancreas.*--Same preparation as for liver.
*Spleen.*--Harden in Müller’s fluid.
Mount one section unstained. Shake another up with water in a test
tube to shew the structure of the pulp. Stain others in eosine and
hæmatoxyline.
*Bladder.*--Must be removed and pinned out immediately after death, as
otherwise the epithelium will be macerated off. Consequently it must be
taken from an animal, as a cat. Harden in osmic acid. Cut in celloidin
as the coats are very apt to become detached.
*Penis* and *testis*.--Readily obtained from dog, cat, or rat.
Stain with eosine and hæmatoxyline.
*Uterus, ovaries, and Fallopian tubes.*--May be obtained from the
post-mortem room or from the lower animals. Harden in Müller’s fluid,
and make sections from the cervix, the body of the uterus, the
Fallopian tube, and the ovary.
Stain with eosine and hæmatoxyline.
*Embryological specimens.*--For systematic work special manuals should
be consulted.
Specimens should be hardened in osmic acid or in Müller’s fluid, and
cut in celloidin, or paraffin.
*Cloudy swelling.*--Specimens are obtained from organs of subjects
who have died in the early stage of some fever. They should be always
hardened in Müller’s fluid, as the appearances alter if the tissue is
kept in spirit for any length of time.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account