It was then thought that perhaps the oil could be converted into an ester which might be more volatile and could be distilled out. 20 grams of the original material were dissolved in 100 cc. of absolute alcohol containing 3 grains of hydrochloric acid gas, and the mixture was heated 10 hours on a water-bath under a return condenser. After the heating, the mixture had a delightful ethereal odor. The flask was corked and left standing several weeks while other work was in progress. The ester solution was then put in a vacuum desiccator over sulphuric acid and the alcohol evaporated. A black, tarry, solid mass was left having the ester odor. It was extracted with warm water and filtered from insoluble tar. The filtrate had a green color and the ethereal odor. It was shaken out with ether; the ether layer had a blood-red color while the water layer was deep green. The extraction with ether was continued until the water layer was no longer green. The combined ether extracts were evaporated in a desiccator without heat. A black tar-like solid was left very much like the original material, but it had the ester odor. It was partly soluble in water and readily soluble in alcohol. The alcoholic solution was tested on the skin and found to be not poisonous. The ester, or mixture of esters, was not investigated further in this connection, but was later shown to give the reactions for gallic acid and methyl furfurol. These reactions will be referred to in connection with other experiments. After a few other preliminary experiments, it became evident that the original material was a complex mixture of substances and that it would have to be fractionated by some means and the fractions studied separately. A portion of the original substance was treated with 50 per cent. alcohol and was found to be partly soluble in this medium. The solution was filtered from insoluble tar. A portion of the yellow filtrate gave a reddish yellow precipitate with lead acetate. The alcoholic solution was distilled in an Anschuetze flask under diminished pressure; a yellow liquid condensed in the arm of the flask while most of the alcohol was collected in a bottle connected with the arm. The yellow liquid was acid to litmus. Water was added, the solution was shaken out with ether and the ether was evaporated. When the small residue was completely dry, it was a yellow solid soluble in dilute alcohol and acid to litmus. The substance was not volatile enough to justify the use of this method for getting it. Chlorophyll could not be removed from the original substance because the solvents for chlorophyll such as alcohol, ether, fats, petroleum, and carbon bisulphide dissolve large quantities of the mixture.