For the benefit of those who have had no experience with the microscope,
I will give the following directions:
Take a small portion of the starch or spice to be examined upon a clean
camel’s hair brush and dust it upon a common slide, blow the excess away
and moisten that retained with a drop of a mixture of equal parts of
glycerine and water, or with glycerine and camphor water, and cover with
a glass. It is well to have a small supply of the common starches in a
series of tubes which can be mounted at any moment and used for
comparison. They may be permanently mounted by making with cork borers,
of two sizes, a wax cell ring equal to the diameter of the cover glass
and, after cementing the cell to the slide with copal varnish thinned
with turpentine and introducing the starch and glycerine mixture, fixing
the cover glass on after running some of the cement over the top of the
ring. A little experience will enable one to put the right amount of
liquid in the cell and to make a preparation that will keep for some
time. After several months, however, it is hard to distinguish the rings
which mark the development of the granule, and it is better to keep it
fresh.
For other purposes, the starches should be mounted in prepared Canada
Balsam, or by well-known methods in which they may be preserved
indefinitely, but they are scarcely visible with ordinary illumination
and must be viewed by polarized light, which will bring out distinctive
characters not seen as well, or not at all, in the other mounts. When
mounted in the manner described, in glycerine and water, or in water
alone, if for temporary use, under a microscope with one objective of
equivalent focus of one-half to one-fifth inch, and with means for
oblique illumination, the starches will display characteristics which
are illustrated in Figs. 2, 3, and 4. The illustrations have been drawn
from Nature; Fig. 2 gives starch stained with iodine; Fig. 3 gives shape
and size of plain starch, and presence or absence of a nucleus, or
hilum, and of the rings and their arrangements which can be made out.
The starch is classed in its proper place.
If mounted in balsam, their appearance is scarcely visible under any
form of illumination with ordinary light, the index refraction of the
granules and the balsam being so similar, but when polarized light is
used the effect is a striking one. (See plates of ginger, where it is
easy to distinguish all the characteristics, except the rings, the
center of the cross being at the nucleus of the granule.)
The principal starches which are met with may be described as follows in
connection with illustrations given, beginning with those of the
arrowroot class, including potato, ginger, and tumeric.
[Illustration:
Fig. 6. & Fig. 7. POTATO STARCH
Fig. 8. MARUNTA STARCH
Fig. 9. & Fig. 10. _Marunta_]
POTATO STARCH
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