There are peculiar difficulties attending experiments on the subject of hearing. An absolutely deaf person may be aware of the sounding of a deep organ note through the sense of feeling, and a well-known experimenter was on the point of drawing interesting conclusions from the behaviour of a spider in response to the notes of a flute, when he found that precisely the same results were obtained by a soundless puff of air. It seems hardly possible to make sure, in the case of a spider in a snare, that the sound vibrations are not _felt_, apart from any sense of hearing, and it is a remarkable fact that it is only the snare-spinning spiders that make any response to sounds:—free-roving spiders are apparently quite deaf. In experimenting with sound we must take two precautions: the instrument used must not necessitate any marked action which may be visible to the spider, nor must it give rise to palpable air-currents. These requirements are best met by a tuning-fork of not too low a pitch. We cannot _feel_ the air vibrations emanating from it, but can only perceive them by the ear, but we have no proof that the spider’s sense of touch ceases precisely at the same point as our own. However, no better instrument for experiment seems to be available, so we take a tuning-fork, and approach it cautiously—in the quiescent state—towards the spider, stationed, we will suppose, in the centre of its snare. No notice is taken, and we carefully withdraw it, set it vibrating, and approach it again in the same manner. There is now generally a response, the spider raising its front legs and extending them in the direction of the fork, or, if the sound is loud, dropping suddenly by a thread and remaining suspended some inches below the snare. The experiment should be repeated several times with the fork sometimes still, sometimes vibrating, and the conclusion arrived at will be that the spider is aware of the vibrating fork—but by which sense? It is noteworthy that a fork giving a low note is always most effective. Now here is a very remarkable fact. In two widely different groups of spiders—the Theraphosidae or so called “bird-eating spiders” and the Theridiidae—there are species with a stridulating or sound-making apparatus, and we should hardly expect a deaf creature to evolve an elaborate mechanism for the production of sound. This is a matter, however, that we shall discuss later. No amount of research has succeeded in localising the sense of hearing in spiders, supposing it to exist. The creature may lose any of its five pairs of limbs (four pairs of legs and one pair of pedipalps) without alteration in its response to sound. If the front legs are missing the second pair are raised when the vibrating fork is approached.