Studies in Spermatogenesis (Part 2 of 2) — John Shaqi
Studies in Spermatogenesis (Part 2 of 2)Stevens, N. M. (Nettie Maria)
Science
Studies in Spermatogenesis (Part 2 of 2)
Stevens, N. M. (Nettie Maria)
Sex; Spermatogenesis
HEMIPTERA HOMOPTERA.
Aphrophora quadrangularis.
The abundance of Aphrophora at Harpswell, Maine, in June and July, 1905,
suggested that it might be well to examine at least one more of the
Hemiptera homoptera for comparison with the many species of Hemiptera
heteroptera which have been recently reexamined by Wilson ('05, '05,
'06).
The larvæ only were collected, as they gave all the desired stages for a
study of the spermatogenesis, and also oögonia and synizesis and
synapsis stages of the oöcytes. In the first collections the testes were
dissected out, but the many free follicles break apart so easily that
the later material was prepared by cutting out the abdominal segments
which contained the reproductive organs, and fixing those without
dissection. The same methods of fixation and staining were employed as
for the Coleoptera. Hermann's safranin-gentian method was especially
effective with this material.
In _Aphrophora_ the follicles of each testis are free, forming a dense
cluster, each follicle being connected with the vas deferens by a short
duct. The very young follicles are spherical, the older ones ovoid in
form. The primary spermatogonia (plate XIV, fig. 237)--very clear cells
with a lobed nucleus which stains slightly--occupy the tip of the
follicle. Next to these comes a layer of cysts of secondary
spermatogonia which are conspicuous for their deeper staining quality
(fig. 238). There appears to be no plasmosome in either class of
spermatogonia. Figure 239 is the equatorial plate of a secondary
spermatogonium. There are 23 chromosomes, two of which are conspicuously
larger than the others and evidently form a pair. The odd one is one of
the three next in size.
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