Studies in Spermatogenesis (Part 2 of 2)Stevens, N. M. (Nettie Maria)
Science
Studies in Spermatogenesis (Part 2 of 2)
Stevens, N. M. (Nettie Maria)
Sex; Spermatogenesis
In various parts of the young male larvæ dividing cells were found and
the number 23 determined (fig. 269). Turning now to the female larvæ to
determine the somatic number, the oögonia proved to be more favorable
for counting. Twenty-four chromosomes were present in equatorial plates
of oögonial mitoses (fig. 270), thus confirming Wilson's results for the
_Anasa_ group of the Hemiptera heteroptera.
In examining sections of female larvæ stained with
safranin-gentian-violet, I was surprised to see a very marked polarized
or bouquet stage and to find among the loops something resembling the
odd chromosome of the growing spermatocytes. It was difficult to get a
clear view of this body as it lay within the loops. In one section of a
slightly earlier stage before synapsis, there were found two pairs of
chromosomes (fig. 271, _x_{1}_, _x_{2}_, and _m_{1}_, _m_{2}_) which
were stained with safranin in contrast with the violet spireme. These
two pairs I interpret as being (1) the homologues of the pair of
_m_-chromosomes, which remain condensed during the growth stage of the
spermatocytes, and (2) a pair of heterochromosomes corresponding to the
odd chromosome of the male. Various combinations of these
heterochromosomes are shown in figures 272-277. Figures 278 and 279 were
taken from mercuro-nitric material stained with iron-hæmatoxylin. In
section 278 the "bouquet" was cut through, showing the bivalent
corresponding to the larger pair in figure 271, and in figure 279 this
element is seen behind the paler loops. The history of these two pairs
of heterochromosomes, which have not, so far as I know, been found
before in oöcytes, should be followed up in older ovaries, and related
species should be examined for similar phenomena.
LEPIDOPTERA.
Cacoecia and Euvanessa.
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