[281] [Prof. Semper also remarks (“Animal Life,” note 47, p. 430) with reference to the Axolotl of Lake Como in the Rocky Mountains, which he states always becomes transformed into _Amblystoma Mavortium_, that this metamorphosis “takes place in the water, and the Amblystomas, so long as they are little, actually live exclusively in the water, as I know by my own experience. A young Amblystoma which I kept alive for a long time, never went out of the water of its own free will, while one nearly twice as large lives entirely on land and only takes a bath now and then. It always goes into the water when the temperature of the air in the cellar, in which my aquaria stand, falls below that of the water--down to about 6° or 8° C.” This statement appears to suggest that the effect of temperature may be a factor in some way concerned in these interesting cases of transformation, and would in any case be well worthy of experimental investigation. Some further details concerning the _Siredon Lichenoides_ of Lake Como have been recently published by Mr. W. E. Carlin (Proc. U.S. National Museum, June, 1881). The lake, which is shallow, is fed by a constant stream of fresh water, but the water of the lake is intensely saline. The Siredon never enter the fresh water stream, but congregate in large numbers in the alkaline waters of the lake. “When about one hundred and fifty were placed in fresh water they seemed to suffer no inconvenience, but it had a remarkable effect in hastening their metamorphosis into the Amblystoma form. Of an equal number kept in fresh water and in the lake water, quite a change occurred with the former after twenty-four hours, while the latter showed no change after several days of captivity. Those that were kept well fed in jars usually began to show a slight change in from two to three weeks, and all of them completed the change into the Amblystoma inside of six weeks, while in some kept, but not specially fed, there were but three changes in three months.” (Nature, Aug. 25th, 1881, p. 388.) R.M.] [282] [Some experiments on the transformation of the Crustacean _Artemia Salina_ into _A. Milhausenii_ by gradually increasing the saltness of the water, and conversely, the transformation of _A. Milhausenii_ into _A. Salina_ by diminishing the saltness of the water, have been made by Schmankewitsch (Zeitschrift f. wiss. Zool. xxv. Suppl. 103 and xxix. 429), but the changes which occur here are much less considerable than in the case of the Axolotl. R.M.] [283] “Reden und kleinere Aufsätze, Th. II.: Studien aus dem Gebiete der Naturwissenschaften.” St. Petersburg, 1876, p. 81.