Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
What, then, we must ask ourselves, is the nature of these particles of
dust? On September 27th, 1872, we picked from a vine, in the
neighbourhood of Arbois, a bunch of grapes, of the variety called _le
noirin_. The bunch selected, without any injury to a single grape, was
brought to our laboratory in a sheet of paper that had been previously
scorched in the flame of a spirit lamp, and the grapes were cut off with
a pair of fine scissors, which had also been passed through the flame.
By means of a badger-hair brush, thoroughly purified in water, each
grape to which a portion of its peduncle remained attached, was washed
in a little pure water. The successive washing of a dozen grapes in 3
c.c. of water was sufficient to make the water turbid; we then examined
it under the microscope. Each field contained many little organized
bodies, accidentally associated, now and again, with some very scarce
crystalline spicules. As a rule, the organisms consisted of simple,
transparent, colourless cells; some, indeed, of larger size had a
yellowish brown colour, and were detached or united in irregular masses;
and, lastly, there were club-shaped or bottle-shaped vessels, full of
spores ready to germinate. We repeated this experiment with bunches of
other varieties of grape, and also submitted to examination water in
which the outer surfaces of gooseberries, plums, and pears had been
washed; the result was in each case the same, that is, we found a great
number of the same colourless cells, and the same irregular masses of
darker cells, which latter, however, we must not confound with the
masses of dead cells sometimes found covering parts of the epidermis of
certain fruits.
As we had purposely left each fruit attached to part of its peduncle, we
wished to ascertain if these corpuscles proceeded from the grapes or
from the wood of the peduncle. For this purpose we washed separately the
surface of the grapes and the woody part of the bunch. The water in
which the latter was washed was visibly more charged with the minute
organisms than that in which the grapes was washed, although the latter
was by no means free from them.
Plate VIII. represents these corpuscles as they exist on the surface of
fruits, magnified 500 times. The groups, _b_, _b_, _b_, ..., _c_, _c_,
... are of a brown colour, more or less dark, or of a reddish yellow;
the cells _a_, _a_, ... are transparent. Amongst them are some spores of
ordinary fungoid growths, and several cells which are probably the issue
of a germination that had commenced in certain groups which have a hard,
yellowish appearance, and which are provided with what seems to be a
double case—_b_, _b_, _b_, ..., _c_, _c_, ..., a result of the moisture
of the woody part of the bunch, or of rain that fell just before the
commencement of our observations.
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