Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
It is an easy matter to trace the germination of these different
varieties of cells with the microscope. We put a drop of the water in
which the woody part of a bunch of grapes has been washed into a small
quantity of wort, previously boiled and filtered bright. Plate IX.
presents a series of developments observed in the case of simple or
grouped cells, A, D, G, and J. The process is as follows: The
yellowish-brown cells soften and grow larger in the nutritive medium,
and gradually become almost transparent and colourless. At the same time
we see some very young buds appear on their margins; these rapidly
increase in size, and detaching themselves to make room for others, move
off as young cells that after a time bud in their turn. The rapidity
with which these cells bud and multiply is often extraordinary. The
group A and the cell D produced the groups C and F within twenty-four
hours, passing through the intermediate stages represented in groups B,
E. The cells A and D did not give rise to any filamentous growths, at
least whilst under our observation. Some groups of cells, however, put
forth, from the first, long filaments, having cross-partitions and
resembling the mycelium in ordinary fungoid growths. Together with
these, and along their whole length, was an abundance of cells, often in
clusters, as represented by Fig. G, the whole of which growth took place
in less than twenty-four hours.[86] But apart from contact with the air,
there was a complete absence of life.
[Illustration: Plate 9. Various Examples of the Mode of Growth of
Mould-cells from the Outer Surface of Grapes.]
The figures H, I, J, K, represent other aspects of developing cells and
filaments. The cells H are spherical; the cells I have numerous buds, as
also have those marked K. These different forms were all produced in the
course of twenty-four hours by the cell which may be observed in the
centre of the group J. In connection with this same group, J, we may
remark that on September 30, 1872, at 10 A.M., we witnessed the
detachment of three oval cells at the points _a_, _b_, _c_; by 10.45
other buds of the size represented in our engraving had formed in their
place; by about five o’clock that same afternoon these buds, _a_, _b_,
_c_, having become transformed into cells, fell off in their turn.[87]
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