Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
The proportions were 1 of yeast to 76 of fermented sugar in the first
case, and 1 of yeast to 89 of fermented sugar in the second.
From these facts the following consequences may be deduced:
1. The fermentable liquid (flask B), which since it had been in contact
with air, necessarily held air in solution, although not to the point of
saturation, inasmuch as it had been once boiled to free it from all
foreign germs, furnished a weight of yeast sensibly greater than that
yielded by the liquid which contained no air at all (flask A), or, at
least, which could only have contained an exceedingly minute quantity.
2. This same slightly aerated fermentable liquid fermented much more
rapidly than the other. In eight or ten days it contained no more sugar;
while the other, after twenty days, still contained an appreciable
quantity.
Is this last fact to be explained by the greater quantity of yeast
formed in B? By no means. At first, when the air has access to the
liquid, much yeast is formed and little sugar disappears, as we shall
prove immediately; nevertheless the yeast formed in contact with the air
is more active than the other. Fermentation is correlative, first to the
development of the globules, and then to the continued life of those
globules once formed. The more oxygen these last globules have at their
disposal during their formation, the more vigorous, transparent, and
turgescent, and, as a consequence of this last quality, the more active
they are in decomposing sugar. We shall revert hereafter to these facts.
3. In the airless flask the proportion of yeast to sugar was 1/89; it
was only 1/76 in the flask which had air at first.
The proportion that the weight of yeast formed bears to the weight of
the sugar is, therefore, variable, and this variation depends, to a
certain extent, upon the presence of air and the possibility of oxygen
being absorbed by the yeast. We shall presently show that yeast
possesses the power of absorbing that gas and emitting carbonic acid,
like ordinary fungi, that even oxygen may be reckoned amongst the number
of food-stuffs that may be assimilated by this plant, and that this
fixation of oxygen in yeast, as well as the oxidations resulting from
it, have the most marked effect on the life of yeast, on the
multiplication of its cells, and on their activity as ferments acting
upon sugar, whether immediately or afterwards, apart from supplies of
oxygen or air.
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