Studies on Fermentation: The diseases of beer, their causes, and the means of preventing themPasteur, Louis
Science
Studies on Fermentation: The diseases of beer, their causes, and the means of preventing them
Pasteur, Louis
Beer; Fermentation
The following are some of the numerous microscopic observations which we
made on the liquids and their deposits.
On June 1st the liquid in one of the six flasks which had begun to
ferment was covered with a continuous scum of _mycoderma vini_, below
which appeared a filamentous network belonging to a _mycelium_, or other
fungoid growth. But neither in the liquid itself nor in the deposit
could we perceive any cells of the ferment of beer; the field, however,
was swarming with active butyric _vibrios_, rather thick and short,
their length being about twice their diameter. This fermentation was
exclusively butyric.
On June 2nd another of the flasks showed no _vibrios_, but alcoholic
ferment in small quantity, much lactic ferment, consisting of little
particles contracted at the middle and non-mobile, and, finally, some
slender filaments, resembling those represented in Plate I., Nos. 1 and
2.
On June 3rd we examined the liquid in the flask which showed the most
marked fermentation. In addition to the flakes of fungoid growths,
checked in their development through want of air, we found at least five
distinct productions, which are represented in the accompanying sketch
(Fig. 11).
_aaa._—Thick cells of alcoholic ferment, the size of which is given
in our sketch: thus, 13/450 indicates that the corresponding figure
is 13/450 millimetre in length (rather more than 1/1000 inch).
_bbb._—Small alcoholic ferment, such as we generally see in the must
of acid and sugared fruits—especially in filtered grape must. Its
dimensions varied from 1 to 1-½, or 2, 450ths of a millimetre.[43]
[Illustration: Fig. 11.]
_ccc._—“Low” yeast, of a type resembling that existing in other
preparations fermenting in the room.
_ddd._—Enlarged, distended spores of _mucor racemosus_. These are
scarce, and have an old appearance. We shall come to them again in a
subsequent chapter, where we shall explain their real significance.
_eee._—Short _vibrios_, occurring either contracted or not near the
middle. Some were motionless, others vibrating to and fro and
executing other movements. These forms belong to the butyric and
lactic ferments shown on Plate I.
The preceding series of experiments shows us that, in the case of wort
exposed to the air, germs of divers organisms, amongst which various
ferments—butyric, lactic, and alcoholic—are to be found, fall
simultaneously from the particles of dust floating in the air. We must
observe, however, that we were dealing with the air of a laboratory in
which we were constantly studying analogous fermentations, and that a
different atmosphere would, most likely, give us different results. We
shall see a proof of this in the following paragraph, where we shall
also find some new facts tending to prove that the germs of alcoholic
ferment do not exist amongst the particles of dust floating in the air,
in anything like the quantity usually supposed.
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