On December 13th, 1872, we sowed the little plant in a state of purity, by means of a piece of platinum wire, in the manner already explained in connection with propagation of other fungoid growths. On December 19th a young and thin film of _mycoderma aceti_ covered the surface of the liquid. We then poured out the liquid through the right-hand tube, at the same time heating the end of the bent tube, to purify the air that passed into the flask. The whole film of _mycoderma aceti_ remained adhering to the interior sides of the flask during this decanting. The question then was how to convey this film of the little plant into a saccharine liquid of a particular kind. We effected this easily by the following means: After having emptied the flask, as just described, instead of re-closing the india-rubber nozzle on the end of the right-hand tube, we attached it to a test-flask containing the saccharine liquid on which we wished to operate. This had been previously boiled in the test-flask, and when we attached the neck of the test-flask, previously slightly drawn out and curved, to the india-rubber tube, the liquid was still very warm. We permitted the liquid in the test-flask to cool down, and, then, taking up the test-flask, we decanted its contents into the other flask, in which, as we have already said, the film of _mycoderma aceti_ had been left. In this way the film became partly submerged, partly spread over the surface of the new liquid. Experiments were made with two saccharine liquids, must and wort. In the case of the latter, from December 22nd the whole surface of the liquid was covered by a film of _mycoderma aceti_, which even spread up the moist sides of the flask above the level of the liquid. In the case of the must, on the other hand, the plant for some time did not seem to be developing; on December 24th, however, it was visibly spreading over the surface of the must. The following days we frequently shook up the films to separate them, and spread them over the subjacent liquid. There were no signs of alcoholic fermentation. On December 30th we introduced several grammes (50 or 60 grains) of carbonate of lime into each of the flasks, an operation of little difficulty, which we effected in a manner similar to that just described. We substituted for the test-flask another flask—or, better still, a simple glass tube—containing carbonate of lime that had been subjected to great heat in the flask or tube, and there left to cool down. When cold, we poured the powdered carbonate of lime into the liquid in the flask, in this way avoiding the possibility of any error from the introduction with the carbonate of lime of any foreign germ.