Development of the Ovules and Pollen in Sweet Clover.
Fig. 1.--Section through the nucellus of an ovule of
_Melilotus alba_, showing two megaspore mother cells. × 360. Fig.
2.--Median section through an ovule of _Melilotus alba_, showing the
two cells resulting from the first division of the megaspore mother
cell, and the relative development of the different parts of the ovule.
× 300. Fig. 3.--Section through the nucellus of an ovule of _Melilotus
alba_, showing two embryo sacs, one being more advanced than the other.
× 360. Fig. 4.--Protoplasm of the pollen mother cell of _Melilotus
alba_ contracted and ready to undergo division. × 560. Fig. 5.--Pollen
grains of _Melilotus alba_ just formed, showing their dense cytoplasm
and the presence of the mother-cell wall. × 560. Fig. 6.--_a_, Mature
pollen grain of _Melilotus alba_, showing the binucleate condition at
the time of shedding; _b_, surface view. × 560.
Plate II.
[Illustration]
Fig. 1.--Median Section through an Ovule of Melilotus alba.
The embryo sac is shown ready for fertilization. The egg and synergids
are in contact with the outer integument at the micropylar end. The
remains of the antipodals may be seen at the chalazal end. × 180.
[Illustration]
Fig. 2.--Section through an Ovule of Melilotus alba, about Three
Days After Fertilization.
The proembryo, the endosperm, and modifications of the integuments are
shown. At this stage the suspensor prominent part of the proembryo, and
the endosperm is most abundant around the embryo. The inner integument
is being rapidly destroyed, and the outer integument is beginning to
form the seed coat, as is indicated by the modifications in the outer
layer of its cells, which are elongating and thickening their outer
walls. × 33.
Plate III.
[Illustration]
Fig. 1.--Section of an Ovule of Melilotus alba after
Fertilization.
The stage of development is a little later than that shown in Plate II,
figure 2. The embryo is embedded deeply in endosperm tissue. × 45.
[Illustration]
Fig. 2.--Section through an Ovule of Melilotus alba after the
Embryo is Nearly Half Mature.
But little endosperm remains except in the chalazal end, and very
little remains of either the nucellus or inner integument. The
modifications which transform the outer integument into a seed coat are
well under way. Not only the outer layer of cells which becomes the
Malpighian layer is quite well modified, but also the layer beneath is
being transformed into the osteosclerid layer. × 30.
Plate IV.
[Illustration]
Stubble of Melilotus alba.
These plants, which were cut 12 inches above the ground during rainy
weather, had made a 40 to 42 inch growth. The stubble became infected
at the top and the light-colored portions of them were killed by
disease, thus checking the water supply to the growing branches above
the infection.
MATURE POLLEN OF SWEET CLOVER.
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