Permeable and impermeable seeds[4] of _Melilotus alba_ and _M.
officinalis_ were obtained from commercial samples and also from
samples collected in the field. Those selected for sectioning were
allowed to dry after being removed from the germinator and then
embedded on the ends of pine blocks in glycerin gum, which was made by
dissolving 10 grams of powdered gum arabic in 10 c. c. of water and
adding 40 drops of glycerin. After the glycerin gum had dried for 24
hours, the seeds were easily sectioned. This method of embedding causes
no change in the seed coat. It is more satisfactory than the paraffin
method for holding the seeds firmly. The glycerin gum dissolved readily
when the sections were mounted in water.
[4] The term "permeable" is used in this paper to designate seeds whose
coats are permeable to water in two weeks or less at temperatures
favorable for germination, while the term "impermeable" is used to
designate seeds whose seed coats are impermeable to water for this
length of time when temperatures are favorable for germination.
Impermeable seeds are commonly referred to as "hard seeds," and they
may become permeable in time.
In the microchemical studies Sudan III, alcanin, chlorophyll solution,
and phosphoric acid iodin were used to test for cutin or suberin;
sulphuric acid and iodin, chloriodid of zinc, and chloriodid of
calcium for cellulose; phloroglucin and hydrochloric acid for lignin;
ruthenium red for pectic substances; and sulphuric acid, Congo red, and
aniline blue for callose.
Where very thin sections were necessary for detailed study of the
structure of the seed coat, pods in various stages of development were
collected, and after the usual preliminary treatment they were embedded
in paraffin and sectioned with the microtome. Microchemical tests were
made with these sections by using various specific stains. Safranin was
used to test for cutin, suberin, and lignin; haematoxylin and methyl
blue for cellulose ; methylene blue, methyl violet B, mauvein, and
ruthenium red for pectic substances; and aniline blue and Congo red for
callose. In studying some points with reference to the pore system of
the seed coat, it was necessary to use free-hand sections of fresh pods.
In studying the seed coat in relation to the absorption of water,
both permeable and impermeable seeds were soaked in water solutions
of safranin, gentian violet, eosin, and haematoxylin, then dried and
embedded in glycerin gum for sectioning. Seeds were soaked in stains
dissolved in 95 per cent alcohol to test the penetration of alcohol. It
was evident that the seed coats did not act as a filter, as the stains
passed through them with the water or alcohol.
STRUCTURE OF THE SEED COAT.
There is very little endosperm present in mature seeds of _Melilotus
alba_ or _M. officinalis_. That which is present is quite permeable to
water and therefore bears no relation to the impermeable seeds of these
plants.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account