Technique of Eye DissectionsWoll, Frederic A. (Frederic Albert)
Science
Technique of Eye Dissections
Woll, Frederic A. (Frederic Albert)
Dissection; Eye -- Surgery
By using the tweezers the cornea may be split. Nothing in the way of
locating its layers can be recognized, however, unless a section is made
for microscopic examination. The epithelial may be scraped off when the
cornea is a trifle dry. This is the ocular epithelium reduced to a layer
of flattened cells.
THE CRYSTALLINE LENS
If the preceding dissections have been done, the crystalline lens will
already have received some notice. To study the lens properly one should
use an eye that has not been hardened and also an eye or the lens of an
eye that has been in a 5 per cent. solution of formaldehyde for about
two weeks.
The lens in the unhardened eye will prove too friable to permit much
handling. The dissection should be made, however, in order to give
opportunity to notice the crystalline clearness of the lens substance,
its great magnifying power, its attachments, its capsule, etc. For this
purpose it is necessary to proceed only as in the dissection for the
“hyaloid membrane, etc.” That is, use an eye that has been kept in a
cool place for several days, and then open it, and remove hyaloid,
vitreous, and lens intact, as in the first dissection taken up in this
book. To examine the specimen in detail, turn it so the lens will be
uppermost. (Fig. 18.)
To remove the lens it is necessary to separate the suspensory ligament,
using for this purpose the small-pointed scissors. The capsule may be
removed by picking it up on the periphery of the lens, and stripping it
off. It will peel off about the same way that the outer skin of a bean
or pea does.
[Illustration: Fig. 18--Enlarged to show the processus zonuloe. (Page
53.)]
The tri-radiate lines on the posterior and the anterior surfaces of the
lens will not be as clearly discernible as in the lens coming from the
hardened eye. Close inspection and the use of a hand lens will help
bring them out more clearly.
Now, with the point of the scalpel try to separate the outer layers
(cortex) from the harder inner layers (nucleus). This will not prove
very successful but is suggested for the purpose of comparison when the
same thing is done to the hardened lens.
It will be found that the lens after having been in the formaldehyde
solution is no longer crystal like, but more or less translucent. When
viewed from either the anterior side or the posterior side, the
tri-radiate lines on each surface will be seen to begin at the poles of
the lens and radiate outward toward the lens equator. Holding the lens
up to a strong light will show that though the lines on either surface
form angles of 120 degrees, the angles formed by the lines on one side
with the lines on the other side are 60 degrees. On the anterior surface
of the lens the vertical line extends upward from the pole; on the
posterior surface downward from the pole.
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