The anatomy of the domestic fowlKaupp, B. F. (Benjamin Franklyn)
Science
The anatomy of the domestic fowl
Kaupp, B. F. (Benjamin Franklyn)
Poultry -- Anatomy
Similar sections in green bone may be studied if prepared as follows.
Secure specimen of bone just removed from a fowl and place it for three
days in a 10 per cent. aqueous solution of hydrochloric acid. Test by
puncturing with a needle, and, if all the mineral salts are removed,
place in a water bath and wash for four hours. Pass it through the
fluids usually employed in preparing specimens for sectioning with the
microtome. Stain as sections of other tissue for microscopic study. See
the description below. If the ends of the bone be included, it will
enable the student to study not only compact bone but also cancellated
bone and articular, or hyaline cartilage, and in some of the bones, as
the femur, the red marrow.
=Special Technic for the Dissection of Cranial and Spinal Nerves.=—It is
rather difficult to dissect the cranial and spinal nerves of the fowl,
owing to the fact that the structures are very small. The bone is rather
hard and the nerve tissue so delicate that great skill must be attained
to achieve any degree of success.
A simple technic has been developed as follows: Place the head and neck,
or other structures of the spinal column in a 10 per cent. aqueous
solution of hydrochloric acid for three or more days, the time depending
on the size of the specimen and the amount of soft structures
surrounding it. This solution removes all the calcium salts from the
bone and makes the removal of the bony structures a less difficult task.
=Directions for the Study of Soft Structures.=—Secure a specimen of the
tissue to be studied—lung, muscle, intestine, liver, pancreas—from a
normal fowl just killed. The specimen should be not more than ½ inch
square. After first hardening three days in 10 per cent. formaldehyde.
Pass through the following fluids:
1. Alcohol, 95 per cent. 24 hours
2. Alcohol, absolute 24 hours
3. Alcohol and ether, equal parts 24 hours
4. 1 per cent. celloidin 24 hours
5. 2 per cent. celloidin 24 hours
6. 4 per cent. celloidin 24 hours
7. 6 per cent. celloidin 24 hours
8. 10 per cent. celloidin 24 hours
9. Place on block, and as soon as solid, place in 80 per cent. alcohol
until ready to section. Histoloid or parlodion will take the place
of celloidin.
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