It is stated[36] that in Servia, Bulgaria,[37] and Roumania there were 5000 centenarians living in 1896, and while many reasons are advanced for such an abnormal condition of affairs, it seems fairly certain that the sole reason why people in these districts live to such great ages is because of their mode of living and the fact that they live very largely on soured milk. The hygienic conditions throughout these countries are not such as would give the population in the towns and villages any special advantages in the prolongation of life, and while it may be stated that a pastoral and agricultural life are likely to contribute to longevity, these conditions would not account for a general tendency to live long in the countries referred to, more than in any other agricultural area. There are many countries throughout the world in which the pastoral and agricultural existence is general, but it has not been shown that in these countries life is prolonged. Hence the conclusion has been forced upon investigators that the reason is to be found not in the pastoral conditions, but in the habit which has existed from time immemorial of consuming sour milk as a principal article of diet. There is no curtailment of the use of fermented milks in Eastern Europe, and the methods of preparation at the present day are those which have been carried out from time immemorial. A local observer states that in Bulgaria yoghourt is made in nearly every household, especially in the spring and summer. The method of preparation is very simple: The milk is boiled until a quarter of its volume has evaporated, it is then cooled to 45° C. and the ferment added. This ferment is a portion of the yoghourt of good flavour and is called "Maya" or "Zakvaska." The vases, a kind of earthenware pot, are enveloped in woollen stuff or sheepskin and placed in a warm place near the chimney. In ten hours the yoghourt is made, and it is preserved in a cold place. The great reputation that the yoghourt has acquired in Western Europe has caused this "Maya" to become an article of commerce. It is sent out by rail hermetically sealed in tinplate boxes. According to a Sophia chemist, the "Maya" is employed in the following manner: For a litre of milk it is necessary to take about 10 gr. of the ferment. This ferment is diluted with three times the amount of water and put into a bowl previously heated with hot water and dried. Into this bowl the milk, previously boiled and cooled to a temperature of 75° to 50° C., is poured; it is then covered over and put in a temperature of about 30° C., and, in default of a stove of constant temperature, the bowl is wrapped round with flannel or a plaid, and left to curdle for eight to ten hours. It is then ready for consumption. During winter, curdled milk keeps for several days, and in summer it becomes sour in from twelve to twenty-four hours.