I am indebted to all of these persons for their aid, and to various
collectors for the Museum of Natural History, who preserved bacula with
specimens. Many of these specimens were obtained through the assistance
of the University of Kansas Endowment Association and the National
Science Foundation.
METHODS
Bacula were obtained from fresh specimens, specimens preserved in alcohol
or formalin, and dried study skins. The processing of bacula has been
discussed by Hamilton (1946), Friley (1947), White (1951), and Dearden
(1958). The methods used to preserve bacula for my study differed some
from any of those reported. The terminal part of each penis including the
baculum imbedded in the glans penis was removed in its entirety and
placed in a vial. The catalogue number was kept with each specimen at all
times. A two per cent solution of potassium hydroxide was added. All
specimens were examined at least once a day. If tissues other than the
glans penis were present they were removed with forceps when softened
usually at the end of one day. Several drops of Alizarin red-S stain in a
saturated alcoholic solution were added to the 3 to 5 ccs. of KOH
solution in each vial. Solutions were replaced if they became turbid
enough to obstruct observation of the clearing penis. After one day the
solution containing stain was removed and replaced with two per cent KOH
solution without stain. When the glans became sufficiently cleared that
the stained baculum could be seen easily, the solution was replaced by
glycerin in which clearing was completed. The time required for the
entire process varied from one day to more than two weeks depending on
the size of the specimen and on its condition. Fresh specimens clear more
rapidly than dried specimens, and those that are dried more rapidly than
those that are preserved. A three or four per cent solution of hydroxide
will hasten the process, but more frequent observation is required to
prevent excessive maceration.
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