This variation not only affects the fat, but all constituents of the milk. While there is a difference in the composition of the milk from cows of different breeds, there is almost as wide variation in the composition of the milk from single cows[2] of the same breed. With the same cow the stage of lactation causes a wide variation in the composition of the milk.[3] As the period of lactation advances, the milk increases in percentage of fat and other solids. +9. Milk constituents.+--From the standpoint of the cheese-maker, the significant constituents of milk are water, fat, casein, milk-sugar, albumin, ash and enzymes. These will be discussed separately. +10. Water.+--The retention of the solids and the elimination of the water are among the chief considerations in cheese-making. Water forms 84 to 89 per cent of milk. Cheese-making calls for the reduction of this percentage to that typical of the particular variety of cheese desired with the least possible loss of milk solids. This final percentage varies from 30 to 70 per cent with the variety of cheese. The water has two uses in the cheese: (1) It imparts smoothness and mellowness to the body of the cheese; (2) it furnishes suitable conditions for the action of the ripening agents. To some extent the water may supplement or even replace fat in its effect on the texture of the cheese. If the cheese is properly made, the water present is in such combination as to give no suggestion of a wet or "leaky" product. +11. Fat.+--Fat is present in the milk in the form of suspended small transparent globules (as an emulsion). These globules vary in size with the breed and individuality of the cow and in color from a very light yellow to a deep yellow shade as sought in butter. Milk with small fat globules is preferred for cheese-making, because these are not so easily lost in the process. Milk-fat is made up of several different compounds called glycerids,[4] which are formed by the union of an organic acid with glycerine as a base. Fat is important in cheese-making for two reasons: (1) Its influence on the yield of cheese; (2) its effect on the quality of the cheese. Many of the details of cheese-making processes have been developed to prevent the loss of fat in manufacture. The yield of cheese is almost directly in proportion to the amount of fat in the milk; nevertheless, because the solids not fat do not increase exactly in proportion to the fat, the cheese yield is not exactly in proportion to the fat. The fat, however, is a good index of the cheese-producing power of the milk.