+22. Acid fermentation of milk.+--By far the most common and important fermentation taking place in milk is due to the action of the lactic acid-forming bacteria on the milk-sugar or lactose. The bacteria that bring about this fermentation may be divided into several groups on the basis of their morphology, proteolytic activity, gas production, temperature adaptation and production of substances other than lactic acid. The larger number of organisms producing lactic acid in milk also produce other organic acids in greater or less abundance. Inasmuch as lactic acid is the principal substance produced, they are called lactic acid organisms. This group contains different kinds of organisms which may be subdivided into small groups as follows: (a) _Bacterium lactis-acidi_ group. (b) _Bacterium colon-aërogenes_ group. (c) Acid peptonizing group. (d) _Bacillus bulgaricus_ group. (e) Acid cocci or weak acid-producing group. +23. Bacterium lactis-acidi group.+--There are many strains or varieties in this group which are closely related in their activities. They are universally present in milk and are commonly the greatest causal agent in its souring. They are widely distributed in nature. At a temperature of 65° F. to 95° F., these bacteria grow and multiply very rapidly; at 70° F. (approximately 20° C.) these forms usually outgrow all others. The total amount of acid produced in milk by these organisms varies from 0.6 of one per cent to 1 per cent acid calculated as pure lactic acid. These forms coagulate milk to a smooth curd of uniform consistency. In addition to the lactic acid, there are produced traces of acetic, succinic, formic and proprionic acids, traces of certain alcohols, aldehydes and esters. Substances other than lactic acid are not produced by organisms of this group to such an extent as to impart undesirable flavors to the milk. The action of this group on the milk proteins is very slight. They produce no visible sign of peptonization. The _B. lactis-acidi_ group of organisms are essential to the production of the initial acidity necessary in most types of cheese. The practical culture and utilization of them for this purpose under factory conditions are discussed in Chapter IV, entitled "Lactic Starters."