+43. The sediment test.+--The presence of solid material or dirt in the milk is always accompanied by bacterial contamination. By means of the sediment test, the amount of solid material can be determined. The test consists of filtering the milk through a layer of cotton; the foreign material is left on the cotton filter. Various devices for filtering the milk have been manufactured. (Figs. 4 and 5.) In order to be able to compare the filters from the different dairy-men's milk, the same amount of each patron's milk is filtered, usually about a pint. These tests are usually made once or twice a month at the factory and the filters placed on a card where the dairy-men can see them. Much improvement in the quality of the milk has been accomplished by the use of the sediment test. The purpose of this test may be and often is defeated by the use of efficient strainers. Milk produced in an unclean way may be rendered nearly free from sediment if carefully strained. It must be remembered that the strainer takes out only the undissolved substances and that bacteria and soluble materials which constitute a very large part of the filth pass through with the milk. CHAPTER III _COAGULATING MATERIALS_ At the present time, two substances are used to coagulate milk for cheese-making,--rennet extract and commercial pepsin.[20] Many substances will coagulate milk, such as acids and other chemicals. Enzymes in certain plants will also coagulate it. The curing or ripening of the cheese seems to depend on the physical and chemical properties of the curd, on the activity of certain organisms and on enzymes produced by them or in the milk. Rennet extract and pepsin are the only known substances which will produce curd of such character as will permit the desired ripening changes to take place. Until recently, rennet extract was principally used to coagulate the milk, but because of the scarcity, pepsin is now being substituted. +44. Ferments.+--Many of the common changes taking place in milk are due to fermentations. The souring of milk is one of the most familiar cases of fermentation. The important change taking place is the formation of lactic acid from the milk-sugar. The change is brought about by certain living organisms, namely, the lactic acid-forming bacteria. Another familiar case of fermentation is the coagulation of milk by rennet extract or pepsin. In this case, the change is produced by a chemical substance, not a living organism. Fermentation may be defined as a chemical change of an organic compound through the action of living organisms or of chemical agents.